PMID- 32932705
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201027
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 8
IP  - 3
DP  - 2020 Sep 12
TI  - Dendritic Cells and Myeloid Derived Suppressor Cells Fully Responsive to 
      Stimulation via Toll-Like Receptor 4 Are Rapidly Induced from Bone-Marrow Cells 
      by Granulocyte-Macrophage Colony-Stimulating Factor.
LID - 10.3390/vaccines8030522 [doi]
LID - 522
AB  - Dendritic cells (DCs) are commonly generated from bone marrow (BM) progenitor 
      cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or in 
      combination with interleukin 4 (IL-4). These cells are often harvested post day 
      5, when they acquire maturation markers and can stimulate T cells. Apart from 
      DCs, myeloid derived suppressor cells (MDSCs) are also found within these 
      cultures. However, little is known about the functional characteristics of DCs 
      and MDSCs before day 5. Herein, using a murine model, it is shown that early DCs 
      and MDSCs, even in cultures with GM-CSF alone, upregulate fully maturation and 
      activation surface molecules in response to the toll-like receptor 4 (TLR4) 
      ligand lipopolysaccharide (LPS) stimulation. Despite initially displaying lower 
      marker expression levels, these cells efficiently induced T cell stimulation and 
      cytokine production. Interestingly, Gr-1(int) MDSCs increased their T cell 
      co-stimulatory activity upon TLR4 stimulation. Additionally, early DCs and MDSCs 
      exhibited differential endocytic capacity for viral sized nanoparticles and 
      bacterial sized microparticles. DCs internalized both particle sizes, whilst 
      MDSCs only internalized the larger microparticles, with reduced endocytic 
      activity over time in the culture. These findings have unveiled an important role 
      for the rapid initiation of productive immunity by GM-CSF, with promising 
      implications for future vaccine and DC immunotherapy developments.
FAU - Kong, Ying Ying
AU  - Kong YY
AD  - Institute for Molecular and Cellular Biology, Agency for Science, Technology and 
      Research (A*STAR), Singapore 138673, Singapore.
FAU - Wilson, Kirsty
AU  - Wilson K
AD  - School of Health and Biomedical Sciences, RMIT, Bundoora, VIC 3083, Australia.
FAU - Apostolopoulos, Vasso
AU  - Apostolopoulos V
AUID- ORCID: 0000-0001-6788-2771
AD  - Institute for Health and Sport, Victoria University, Melbourne, VIC 3011, 
      Australia.
FAU - Plebanski, Magdalena
AU  - Plebanski M
AUID- ORCID: 0000-0001-6889-3667
AD  - School of Health and Biomedical Sciences, RMIT, Bundoora, VIC 3083, Australia.
LA  - eng
PT  - Journal Article
DEP - 20200912
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC7564202
OTO - NOTNLM
OT  - GM-CSF
OT  - MDSCs
OT  - bone marrow culture
OT  - dendritic cells
OT  - granulocyte-macrophage colony-stimulating factor
OT  - myeloid derived suppressor cells
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/17 06:00
MHDA- 2020/09/17 06:01
PMCR- 2020/09/12
CRDT- 2020/09/16 01:00
PHST- 2020/09/01 00:00 [received]
PHST- 2020/09/07 00:00 [accepted]
PHST- 2020/09/16 01:00 [entrez]
PHST- 2020/09/17 06:00 [pubmed]
PHST- 2020/09/17 06:01 [medline]
PHST- 2020/09/12 00:00 [pmc-release]
AID - vaccines8030522 [pii]
AID - vaccines-08-00522 [pii]
AID - 10.3390/vaccines8030522 [doi]
PST - epublish
SO  - Vaccines (Basel). 2020 Sep 12;8(3):522. doi: 10.3390/vaccines8030522.