PMID- 32938466
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20220531
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 11
IP  - 1
DP  - 2020 Sep 16
TI  - Human umbilical cord-derived mesenchymal stem cells ameliorate insulin resistance 
      via PTEN-mediated crosstalk between the PI3K/Akt and Erk/MAPKs signaling pathways 
      in the skeletal muscles of db/db mice.
PG  - 401
LID - 10.1186/s13287-020-01865-7 [doi]
LID - 401
AB  - BACKGROUND: Globally, 1 in 11 adults have diabetes mellitus, and 90% of the cases 
      are type 2 diabetes mellitus. Insulin resistance is a central defect in type 2 
      diabetes mellitus, and although multiple drugs have been developed to ameliorate 
      insulin resistance, the limitations and accompanying side effects cannot be 
      ignored. Thus, more effective methods are required to improve insulin resistance. 
      METHODS: In the current study, db/m and db/db mice were injected with human 
      umbilical cord-derived mesenchymal stem cells (HUC-MSCs) via tail vein injection, 
      intraperitoneal injection, and skeletal muscle injection. Body weight, fasting 
      blood glucose, and the survival rates were monitored. Furthermore, the 
      anti-insulin resistance effects and potential mechanisms of transplanted HUC-MSCs 
      were investigated in db/db mice in vivo. RESULTS: The results showed that HUC-MSC 
      transplantation by skeletal muscle injection was safer compared with tail vein 
      injection and intraperitoneal injection, and the survival rate reached 100% in 
      the skeletal muscle injection transplanted mice. HUC-MSCs can stabilize 
      localization and differentiation in skeletal muscle tissue and significantly 
      ameliorate insulin resistance. Potential regulatory mechanisms are associated 
      with downregulation of inflammation, regulating the balance between PI3K/Akt and 
      ERK/MAPK signaling pathway via PTEN, but was not associated with the IGF-1/IGF-1R 
      signaling pathway. CONCLUSIONS: These results suggest HUC-MSC transplantation may 
      be a novel therapeutic direction to prevent insulin resistance and increase 
      insulin sensitivity, and skeletal muscle injection was the safest and most 
      effective way.
FAU - Chen, Guang
AU  - Chen G
AD  - Department of Basic Medical Sciences, Taizhou University Hospital, Taizhou 
      University, No 1139 Shifu Road, Jiaojiang District, Taizhou, 318000, China.
AD  - Department of Basic Medical Sciences, Jiamusi University, No 148 Xuefu road, 
      Xiangyang District, Jiamusi, 154007, China.
FAU - Fan, Xiao-Yan
AU  - Fan XY
AD  - Department of Basic Medical Sciences, Taizhou University Hospital, Taizhou 
      University, No 1139 Shifu Road, Jiaojiang District, Taizhou, 318000, China.
FAU - Zheng, Xiao-Peng
AU  - Zheng XP
AD  - Department of Basic Medical Sciences, Taizhou University Hospital, Taizhou 
      University, No 1139 Shifu Road, Jiaojiang District, Taizhou, 318000, China.
FAU - Jin, Yue-Lei
AU  - Jin YL
AD  - Department of Basic Medical Sciences, Taizhou University Hospital, Taizhou 
      University, No 1139 Shifu Road, Jiaojiang District, Taizhou, 318000, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Jilin Tuhua Bioengineering Company Limited, Shiling Town, Tiedong District, 
      Siping, Jilin, 136000, China.
FAU - Liu, Shuang-Chun
AU  - Liu SC
AD  - Municipal Hospital Affiliated to Medical School of Taizhou University, No 381, 
      Zhongshan east road, Jiaojiang district, Taizhou, 318000, China. 
      dachun3065@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200916
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2
MH  - Humans
MH  - *Insulin Resistance
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Muscle, Skeletal/*physiology
MH  - PTEN Phosphohydrolase/genetics
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Signal Transduction
MH  - Umbilical Cord/cytology
PMC - PMC7493876
OTO - NOTNLM
OT  - Db/db mice
OT  - Erk/MAPK
OT  - HUC-MSCs
OT  - Insulin resistant
OT  - PI3K/Akt
OT  - PTEN, crosstalk
COIS- The authors declare that they have no competing interests.
EDAT- 2020/09/18 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/09/16
CRDT- 2020/09/17 05:31
PHST- 2020/02/18 00:00 [received]
PHST- 2020/07/30 00:00 [accepted]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/09/17 05:31 [entrez]
PHST- 2020/09/18 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/09/16 00:00 [pmc-release]
AID - 10.1186/s13287-020-01865-7 [pii]
AID - 1865 [pii]
AID - 10.1186/s13287-020-01865-7 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2020 Sep 16;11(1):401. doi: 10.1186/s13287-020-01865-7.