PMID- 32939029
OWN - NLM
STAT- MEDLINE
DCOM- 20201214
LR  - 20231112
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Sep 16
TI  - Ocular surface inflammation induces de novo expression of substance P in the 
      trigeminal primary afferents with large cell bodies.
PG  - 15210
LID - 10.1038/s41598-020-72295-x [doi]
LID - 15210
AB  - We evaluated the changes in substance P (SP)-expressing trigeminal neurons (TNs) 
      innervating the cornea following ocular surface inflammation. Ocular surface 
      inflammation was induced in Sprague-Dawley rats using 0.1% benzalkonium chloride 
      (BAK). The corneal staining score, corneal epithelial apoptosis, conjunctival 
      goblet cells, and density of corneal subbasal nerve plexus (SNP) were assessed, 
      and the mRNA levels of SP, interleukin (IL)-1beta, IL-6, and tumour necrosis 
      factor-alpha were measured in corneas and ipsilateral trigeminal ganglia (TG). 
      SP-immunoreactivity (IR) was measured in corneal intraepithelial nerves and TNs. 
      The cell size of corneal TNs in the TG was calculated. All parameters were 
      observed immediately (BAK group), at 1 week (1 w group), and 2 months (2 m group) 
      after 2 weeks of BAK application. BAK caused an increase in the corneal staining 
      score and the number of apoptotic cells, loss of conjunctival goblet cells, 
      reduced density of corneal SNP, and upregulated expression of SP and inflammatory 
      cytokines in both the cornea and TG in the BAK group but those changes were not 
      observed in the 2 m group. On the other hand, SP-IR% and mean cell size of 
      corneal TNs increased significantly in the BAK, 1 w, and 2 m groups, compared to 
      the control. Our data suggest that following ocular surface inflammation, 
      large-sized corneal TNs which normally do not express SP, expressed it and this 
      phenotype switching lasted even after the inflammation disappeared. Long-lasting 
      phenotypic switch, as well as changes in the expression level of certain 
      molecules should be addressed in future studies on the mechanism of corneal 
      neuropathic pain.
FAU - Byun, Yong-Soo
AU  - Byun YS
AD  - Department of Ophthalmology and Visual Science, College of Medicine, Seoul St. 
      Mary's Hospital, The Catholic University of Korea, Banpo-daero 222, Seocho-gu, 
      Seoul, 06591, Republic of Korea. mdbyun@catholic.ac.kr.
AD  - Catholic Institute of Visual Science, The Catholic University of Korea, Seoul, 
      Republic of Korea. mdbyun@catholic.ac.kr.
FAU - Mok, Jee-Won
AU  - Mok JW
AD  - CK St. Mary's Eye Center, Seoul, Republic of Korea.
FAU - Chung, So-Hyang
AU  - Chung SH
AD  - Department of Ophthalmology and Visual Science, College of Medicine, Seoul St. 
      Mary's Hospital, The Catholic University of Korea, Banpo-daero 222, Seocho-gu, 
      Seoul, 06591, Republic of Korea.
AD  - Catholic Institute of Visual Science, The Catholic University of Korea, Seoul, 
      Republic of Korea.
FAU - Kim, Hyun-Seung
AU  - Kim HS
AD  - Department of Ophthalmology and Visual Science, College of Medicine, Seoul St. 
      Mary's Hospital, The Catholic University of Korea, Banpo-daero 222, Seocho-gu, 
      Seoul, 06591, Republic of Korea.
AD  - Catholic Institute of Visual Science, The Catholic University of Korea, Seoul, 
      Republic of Korea.
FAU - Joo, Choun-Ki
AU  - Joo CK
AD  - CK St. Mary's Eye Center, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200916
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Benzalkonium Compounds)
RN  - 0 (IL1B protein, rat)
RN  - 0 (Il6 protein, rat)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Benzalkonium Compounds/*adverse effects
MH  - Cell Body/metabolism
MH  - Conjunctivitis/chemically induced/*genetics/metabolism
MH  - Disease Models, Animal
MH  - Epithelium, Corneal/cytology/drug effects/metabolism
MH  - Goblet Cells/cytology/drug effects/metabolism
MH  - Interleukin-1beta/genetics
MH  - Interleukin-6/genetics
MH  - Keratitis/chemically induced/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Substance P/*genetics/metabolism
MH  - Trigeminal Ganglion/*metabolism
MH  - Tumor Necrosis Factor-alpha/genetics
PMC - PMC7494893
COIS- The authors declare no competing interests.
EDAT- 2020/09/18 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/09/16
CRDT- 2020/09/17 05:41
PHST- 2020/05/12 00:00 [received]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/09/17 05:41 [entrez]
PHST- 2020/09/18 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/09/16 00:00 [pmc-release]
AID - 10.1038/s41598-020-72295-x [pii]
AID - 72295 [pii]
AID - 10.1038/s41598-020-72295-x [doi]
PST - epublish
SO  - Sci Rep. 2020 Sep 16;10(1):15210. doi: 10.1038/s41598-020-72295-x.