PMID- 32940554
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20210719
IS  - 1532-2297 (Electronic)
IS  - 1082-6068 (Linking)
VI  - 51
IP  - 4
DP  - 2021
TI  - Isolation of bioactive components with soluble epoxide hydrolase inhibitory 
      activity from Stachys sieboldii MiQ. by ultrasonic-assisted extraction optimized 
      using response surface methodology.
PG  - 395-404
LID - 10.1080/10826068.2020.1821217 [doi]
AB  - Stachys sieboldii MiQ (SSM) is an important food and medicinal herb in Korea, 
      used to improve memory of patients with senile dementia and cardiovascular 
      diseases. However, little information on bioactive components from SSM or 
      standardized extraction methods for these components is available. This study 
      isolated and purified major components from SSM for the first time, and assessed 
      their ability to inhibit soluble epoxide hydrolase (sEH). The results showed that 
      acteoside is the most potent inhibitor of sEH, with an IC(50) of 33.5 +/- 0.5 muM. 
      Additional active components, including harpagide, tryptophan, and 
      8-acetate-harpagide, along with acteoside, were tentatively identified using 
      high-performance liquid chromatography photodiode array tandem mass spectrometry 
      (HPLC-PDA-MS/MS) and quantified using an ultraviolet detector at 210 nm. Further, 
      an ultrasonic-assisted extraction technique for extraction of four bioactive 
      compounds in SSM was developed and optimized using response surface methodology 
      (RSM). The optimal extraction conditions were: extraction time, 30.46 minutes; 
      extraction temperature, 67.95  degrees C, and methanol concentration 53.85%. The 
      prediction model of RSM was validated with laboratory experiments. The similarity 
      between predicted and actual values was 97.84%. The extraction method is thus a 
      rapid, environment-friendly, energy-saving method can be applied to extract 
      bioactive components from SSM in large quantities.
FAU - Gao, Dan
AU  - Gao D
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Le Ba, Vinh
AU  - Le Ba V
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
AD  - Institute of Marine Biochemistry (IMBC), Vietnam Academic of Science and 
      Technology (VAST), Hanoi, Vietnam.
FAU - Rustam, Rustamov
AU  - Rustam R
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Cho, Chong Woon
AU  - Cho CW
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Yang, Seo Young
AU  - Yang SY
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Su, Xiang Dong
AU  - Su XD
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Kim, Young Ho
AU  - Kim YH
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
FAU - Kang, Jong Seong
AU  - Kang JS
AD  - College of Pharmacy, Chungnam National University, Daejeon, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20200917
PL  - England
TA  - Prep Biochem Biotechnol
JT  - Preparative biochemistry & biotechnology
JID - 9607037
RN  - 0 (Glucosides)
RN  - 0 (Iridoid Glycosides)
RN  - 0 (Phenols)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - 0 (Pyrans)
RN  - 3TGX09BD5B (acteoside)
RN  - 8DUH1N11BX (Tryptophan)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - OF59XHX7SR (harpagide)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Epoxide Hydrolases/*antagonists & inhibitors/*chemistry
MH  - Glucosides/isolation & purification/pharmacology
MH  - Inhibitory Concentration 50
MH  - Iridoid Glycosides/isolation & purification/pharmacology
MH  - Liquid-Liquid Extraction/*methods
MH  - Methanol/chemistry
MH  - *Models, Statistical
MH  - Phenols/isolation & purification/pharmacology
MH  - Phytochemicals/*isolation & purification/*pharmacology
MH  - Plant Extracts/*isolation & purification/*pharmacology
MH  - Pyrans/isolation & purification/pharmacology
MH  - Solubility
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Stachys/*chemistry
MH  - Tandem Mass Spectrometry/methods
MH  - Temperature
MH  - Tryptophan/isolation & purification/pharmacology
MH  - Ultrasonic Waves
OTO - NOTNLM
OT  - HPLC-ESI-MS/MS
OT  - Stachys sieboldii MiQ
OT  - response surface methodology
OT  - sEH activity
EDAT- 2020/09/18 06:00
MHDA- 2021/07/20 06:00
CRDT- 2020/09/17 12:15
PHST- 2020/09/18 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
PHST- 2020/09/17 12:15 [entrez]
AID - 10.1080/10826068.2020.1821217 [doi]
PST - ppublish
SO  - Prep Biochem Biotechnol. 2021;51(4):395-404. doi: 10.1080/10826068.2020.1821217. 
      Epub 2020 Sep 17.