PMID- 32942098
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20201201
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 207
DP  - 2021 Jan 1
TI  - Altered gut microbiome accompanying with placenta barrier dysfunction programs 
      pregnant complications in mice caused by graphene oxide.
PG  - 111143
LID - S0147-6513(20)30982-9 [pii]
LID - 10.1016/j.ecoenv.2020.111143 [doi]
AB  - The wide use of graphene oxide (GO) has raised increasing concerns about the 
      potential risks to environmental and human health. Recent studies have shown the 
      vital role of gut microbiome in various pathological status or even exogenous 
      exposure, but more detailed understanding about the effects of possible gut 
      microbiome alterations under GO exposure on reproductive toxicology evaluations 
      in pregnant mammals remained elusive. Here we found that orally administrated GO 
      daily during gestational day (GD) 7-16 caused dose-dependent pregnant 
      complications of mice on the endpoint (GD19), including decreased weight of dam 
      and live fetus, high rate of resorbed embryos and dead fetus, and skeletal 
      development retardation. Meanwhile in placenta tissues of pregnant mice exposed 
      to GO at dose over 10 mg/kg, the expression levels of tight junctions (Claudin1 
      and Occludin) and vascular endothelial growth factor (VEGFA) decreased 
      approximately by 30%-80%, meaning impaired placenta barrier. According to the 
      data of fecal 16s RNA sequencing in 40 mg/kg dose group and the control group, 
      gut microbiome showed dramatically decreased alpha- and beta-diversity, and upregulated 
      Firmicutes/Bacteroidetes ratio owing to GO exposure. What's more, significantly 
      differentiated abundance of Euryarchaeota is expected to be a special biomarker 
      for failed pregnancy caused by GO. Notably, the result of Spearman correlation 
      analysis suggested that there was a strong link (correlation coefficient>0.6) 
      between perturbed gut microbiome with both abnormally expressed factors of 
      placenta barrier and adverse pregnant outcomes. In summary, the damages of GO 
      exposure to placenta barrier and pregnancy were dose-dependent. And GO exposure 
      was responsible for gut microbiome dysbiosis in mice with pregnant complications. 
      These findings could provide referable evidence to evaluate reproductive risk of 
      GO to mammals.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - Liu, Xiaojing
AU  - Liu X
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, 250012, PR China.
FAU - Zhang, Fengmei
AU  - Zhang F
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, 250012, PR China.
FAU - Wang, Zengjin
AU  - Wang Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, 250012, PR China.
FAU - Zhang, Tongchao
AU  - Zhang T
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, 250012, PR China.
FAU - Teng, Chuanfeng
AU  - Teng C
AD  - School of Chemistry and Chemical Engineering, Shandong University, Qingdao, 
      266237, PR China.
FAU - Wang, Zhiping
AU  - Wang Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, 250012, PR China. 
      Electronic address: zhipingw@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200915
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Occludin)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Animals
MH  - Bacteroidetes
MH  - Dysbiosis
MH  - Feces
MH  - Female
MH  - Fetus
MH  - Firmicutes
MH  - Gastrointestinal Microbiome/*drug effects
MH  - Graphite/*toxicity
MH  - Humans
MH  - Mice
MH  - Occludin/metabolism
MH  - Placenta/metabolism/*physiology
MH  - Pregnancy
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - Graphene oxide
OT  - Gut microbiome
OT  - Placenta barrier
OT  - Pregnant complication
EDAT- 2020/09/18 06:00
MHDA- 2020/12/02 06:00
CRDT- 2020/09/17 20:15
PHST- 2020/04/15 00:00 [received]
PHST- 2020/08/04 00:00 [revised]
PHST- 2020/08/06 00:00 [accepted]
PHST- 2020/09/18 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/09/17 20:15 [entrez]
AID - S0147-6513(20)30982-9 [pii]
AID - 10.1016/j.ecoenv.2020.111143 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2021 Jan 1;207:111143. doi: 10.1016/j.ecoenv.2020.111143. 
      Epub 2020 Sep 15.