PMID- 32943709
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20240429
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Sep 17
TI  - Transcriptomic sex differences in sensory neuronal populations of mice.
PG  - 15278
LID - 10.1038/s41598-020-72285-z [doi]
LID - 15278
AB  - Many chronic pain conditions show sex differences in their epidemiology. This 
      could be attributed to sex-dependent differential expression of genes (DEGs) 
      involved in nociceptive pathways, including sensory neurons. This study aimed to 
      identify sex-dependent DEGs in estrous female versus male sensory neurons, which 
      were prepared by using different approaches and ganglion types. RNA-seq on 
      non-purified sensory neuronal preparations, such as whole dorsal root ganglion 
      (DRG) and hindpaw tissues, revealed only a few sex-dependent DEGs. Sensory neuron 
      purification increased numbers of sex-dependent DEGs. These DEG sets were 
      substantially influenced by preparation approaches and ganglion types [DRG vs 
      trigeminal ganglia (TG)]. Percoll-gradient enriched DRG and TG neuronal fractions 
      produced distinct sex-dependent DEG groups. We next isolated a subset of sensory 
      neurons by sorting DRG neurons back-labeled from paw and thigh muscle. These 
      neurons have a unique sex-dependent DEG set, yet there is similarity in 
      biological processes linked to these different groups of sex-dependent DEGs. 
      Female-predominant DEGs in sensory neurons relate to inflammatory, synaptic 
      transmission and extracellular matrix reorganization processes that could 
      exacerbate neuro-inflammation severity, especially in TG. Male-selective DEGs 
      were linked to oxidative phosphorylation and protein/molecule metabolism and 
      production. Our findings catalog preparation-dependent sex differences in 
      neuronal gene expressions in sensory ganglia.
FAU - Mecklenburg, Jennifer
AU  - Mecklenburg J
AD  - Department of Endodontics, University of Texas Health Science Center at San 
      Antonio (UTHSCSA), San Antonio, TX, 78229, USA.
FAU - Zou, Yi
AU  - Zou Y
AD  - Greehey Children's Cancer Research Institute, UTHSCSA, San Antonio, TX, USA.
FAU - Wangzhou, Andi
AU  - Wangzhou A
AD  - Department of Neuroscience and Center for Advanced Pain Studies, University of 
      Texas at Dallas School of Behavioral and Brain Sciences, Richardson, TX, 75080, 
      USA.
FAU - Garcia, Dawn
AU  - Garcia D
AD  - Greehey Children's Cancer Research Institute, UTHSCSA, San Antonio, TX, USA.
FAU - Lai, Zhao
AU  - Lai Z
AD  - Greehey Children's Cancer Research Institute, UTHSCSA, San Antonio, TX, USA.
AD  - Department of Molecular Medicine, University of Texas Health Science Center at 
      San Antonio (UTHSCSA), San Antonio, TX, 78229, USA.
FAU - Tumanov, Alexei V
AU  - Tumanov AV
AD  - Departments of Microbiology, Immunology & Molecular Genetics, University of Texas 
      Health Science Center at San Antonio (UTHSCSA), San Antonio, TX, 78229, USA.
FAU - Dussor, Gregory
AU  - Dussor G
AD  - Department of Neuroscience and Center for Advanced Pain Studies, University of 
      Texas at Dallas School of Behavioral and Brain Sciences, Richardson, TX, 75080, 
      USA.
FAU - Price, Theodore J
AU  - Price TJ
AUID- ORCID: 0000-0002-6971-6221
AD  - Department of Neuroscience and Center for Advanced Pain Studies, University of 
      Texas at Dallas School of Behavioral and Brain Sciences, Richardson, TX, 75080, 
      USA.
FAU - Akopian, Armen N
AU  - Akopian AN
AD  - Department of Endodontics, University of Texas Health Science Center at San 
      Antonio (UTHSCSA), San Antonio, TX, 78229, USA. Akopian@UTHSCSA.edu.
AD  - Department of Pharmacology, The School of Dentistry, University of Texas Health 
      Science Center at San Antonio (UTHSCSA), 7703 Floyd Curl Drive, San Antonio, TX, 
      78229-3900, USA. Akopian@UTHSCSA.edu.
LA  - eng
GR  - R01 DE017696/DE/NIDCR NIH HHS/United States
GR  - NS104200/NS/NINDS NIH HHS/United States
GR  - NS065926/NS/NINDS NIH HHS/United States
GR  - R01 NS112263/NS/NINDS NIH HHS/United States
GR  - S10 OD021805/OD/NIH HHS/United States
GR  - R01 NS065926/NS/NINDS NIH HHS/United States
GR  - R01 DE029187/DE/NIDCR NIH HHS/United States
GR  - NS102161/NS/NINDS NIH HHS/United States
GR  - R01 NS104200/NS/NINDS NIH HHS/United States
GR  - NS112263/NS/NINDS NIH HHS/United States
GR  - S10OD021805/NH/NIH HHS/United States
GR  - P30 CA054174/CA/NCI NIH HHS/United States
GR  - R01 NS102161/NS/NINDS NIH HHS/United States
GR  - DE029187/NH/NIH HHS/United States
GR  - R01 GM112747/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200917
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Female
MH  - Ganglia, Spinal/physiology
MH  - Gene Expression/genetics
MH  - Inflammation/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oxidative Phosphorylation
MH  - Sensory Receptor Cells/*physiology
MH  - Sex Characteristics
MH  - Transcriptome/*genetics
MH  - Trigeminal Ganglion/physiology
PMC - PMC7499251
COIS- The authors declare no competing interests.
EDAT- 2020/09/19 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/09/17
CRDT- 2020/09/18 05:45
PHST- 2019/08/29 00:00 [received]
PHST- 2020/08/24 00:00 [accepted]
PHST- 2020/09/18 05:45 [entrez]
PHST- 2020/09/19 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/09/17 00:00 [pmc-release]
AID - 10.1038/s41598-020-72285-z [pii]
AID - 72285 [pii]
AID - 10.1038/s41598-020-72285-z [doi]
PST - epublish
SO  - Sci Rep. 2020 Sep 17;10(1):15278. doi: 10.1038/s41598-020-72285-z.