PMID- 32945496
OWN - NLM
STAT- MEDLINE
DCOM- 20210503
LR  - 20211001
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 22
IP  - 4
DP  - 2020 Oct
TI  - Baicalin alleviates collagen‑induced arthritis and suppresses TLR2/MYD88/NF‑kappaB 
      p65 signaling in rats and HFLS‑RAs.
PG  - 2833-2841
LID - 10.3892/mmr.2020.11369 [doi]
AB  - Baicalin is a flavonoid isolated from the root of Scutellaria baicalensis with 
      anti‑inflammatory, antioxidant and antiapoptotic pharmacological properties. 
      however, the therapeutic effect of baicalin on rheumatoid arthritis (RA) is not 
      completely understood. The present study aimed to explore the therapeutic 
      potential and mechanisms underlying baicalin in collagen‑induced arthritis (CIA) 
      model rats. CIA was induced in male SD rats. The hind paw thickness and severity 
      of joint injury were monitored to assess the onset of arthritis. At 28 days after 
      the initial immunization, different doses of baicalin were administered once 
      daily via oral gavage for 40 days. The radiologic and pathological alterations 
      were examined using X‑ray, and hematoxylin and eosin staining, respectively. 
      ELISA was employed to measure the serum levels of proinflammatory cytokines. 
      Reverse transcription‑quantitative PCR and western blotting were conducted to 
      determine the expression of toll‑like receptor (TLR)2, myeloid differentiation 
      factor 88 (MYD88) and NF‑kappaB p65. Baicalin treatment noticeably alleviated 
      radiographic and histologic abnormalities in the hind paw joints of CIA model 
      rats in a dose‑dependent manner. The serum levels of proinflammatory cytokines 
      were significantly decreased in baicalin‑treated CIA model rats compared with 
      vehicle‑treated CIA model rats. The mRNA expression levels of TLR2 and MYD88, as 
      well as the protein expression levels of TLR2, MYD88 and NF‑kappaB p65 were 
      significantly decreased by baicalin treatment in the synovial tissue of CIA model 
      rats and human RA fibroblast‑like synoviocytes. The results suggested that 
      baicalin may exert a beneficial effect on CIA, which may be mediated by 
      inhibiting the TLR2/MYD88/NF‑kappaB signaling pathway.
FAU - Bai, Lin
AU  - Bai L
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Bai, Ya
AU  - Bai Y
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Yang, Yuxin
AU  - Yang Y
AD  - Department of Clinical Medicine, Medical College, Yanbian University, Yanji, 
      Jilin 133002, P.R. China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Huang, Ling
AU  - Huang L
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Ma, Rui
AU  - Ma R
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Duan, Haizheng
AU  - Duan H
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
FAU - Wan, Qiaofeng
AU  - Wan Q
AD  - Department of Pathogenic Biology and Immunology, College of Basic Medical 
      Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200728
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cytokines)
RN  - 0 (Flavonoids)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Plant Extracts)
RN  - 0 (RELA protein, human)
RN  - 0 (Rela protein, rat)
RN  - 0 (Scutellaria baicalensis extract)
RN  - 0 (TLR2 protein, human)
RN  - 0 (Tlr2 protein, rat)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Transcription Factor RelA)
RN  - 347Q89U4M5 (baicalin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*administration & dosage
MH  - Arthritis, Experimental/*chemically induced/*drug therapy
MH  - Arthritis, Rheumatoid/*metabolism/pathology
MH  - Cell Line
MH  - Collagen/*adverse effects
MH  - Cytokines/blood
MH  - Disease Models, Animal
MH  - Flavonoids/*administration & dosage
MH  - Humans
MH  - Male
MH  - Myeloid Differentiation Factor 88/*metabolism
MH  - Phytotherapy/*methods
MH  - Plant Extracts/*administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Scutellaria baicalensis/chemistry
MH  - Signal Transduction/*drug effects
MH  - Synoviocytes/*drug effects/metabolism
MH  - Toll-Like Receptor 2/*metabolism
MH  - Transcription Factor RelA/*metabolism
PMC - PMC7453616
OTO - NOTNLM
OT  - baicalin
OT  - CIA
OT  - TLR2
OT  - MYD8
OT  - NF‑ kappaB p65
OT  - proinflammatory cytokines
EDAT- 2020/09/19 06:00
MHDA- 2021/05/04 06:00
PMCR- 2020/07/28
CRDT- 2020/09/18 08:43
PHST- 2019/12/19 00:00 [received]
PHST- 2020/07/02 00:00 [accepted]
PHST- 2020/09/19 06:00 [pubmed]
PHST- 2021/05/04 06:00 [medline]
PHST- 2020/09/18 08:43 [entrez]
PHST- 2020/07/28 00:00 [pmc-release]
AID - MMR-22-04-2833 [pii]
AID - 10.3892/mmr.2020.11369 [doi]
PST - ppublish
SO  - Mol Med Rep. 2020 Oct;22(4):2833-2841. doi: 10.3892/mmr.2020.11369. Epub 2020 Jul 
      28.