PMID- 32945857
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 1567-1364 (Electronic)
IS  - 1567-1356 (Linking)
VI  - 20
IP  - 7
DP  - 2020 Oct 15
TI  - Cell death in Ustilago maydis: comparison with other fungi and the effect of 
      metformin and curcumin on its chronological lifespan.
LID - foaa051 [pii]
LID - 10.1093/femsyr/foaa051 [doi]
AB  - Ustilago maydis is a Basidiomycota fungus, in which very little is known about 
      its mechanisms of cell survival and death. To date, only the role of 
      metacaspase1, acetate and hydrogen peroxide as inducers of cell death has been 
      investigated. In the present work, we analyzed the lifespan of U. maydis compared 
      with other species like Sporisorium reilianum, Saccharomyces cerevisiae and 
      Yarrowia lipolytica, and we observed that U. maydis has a minor lifespan. We 
      probe the addition of low concentrations metformin and curcumin to the culture 
      media, and we observed that both prolonged the lifespan of U. maydis, a result 
      observed for the first time in a phytopathogen fungus. However, higher 
      concentrations of curcumin were toxic for the cells, and interestingly induced 
      the yeast-to-mycelium dimorphic transition. The positive effect of metformin and 
      curcumin appears to be related to an inhibition of the mechanistic Target of 
      Rapamycin (mTOR) pathway, increase expression of autophagy genes and reducing of 
      reactive oxygen species. These data indicate that U. maydis may be a eukaryotic 
      model organism to elucidate the molecular mechanism underlying apoptotic and 
      necrosis pathways, and the lifespan increase caused by metformin and curcumin.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.
FAU - Soberanes-Gutierrez, Cinthia V
AU  - Soberanes-Gutierrez CV
AD  - Laboratorio de Ciencias Agrogenomicas, de la Escuela Nacional de Estudios 
      Superiores Unidad Leon, Universidad Nacional Autonoma de Mexico, Blv. UNAM 2011, 
      Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Leon 
      Gto., Mexico.
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Km. 9.6 Libramiento 
      Norte Carr. Irapuato-Leon 36824 Irapuato Gto., Mexico.
FAU - Leon-Ramirez, Claudia
AU  - Leon-Ramirez C
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Km. 9.6 Libramiento 
      Norte Carr. Irapuato-Leon 36824 Irapuato Gto., Mexico.
FAU - Sanchez-Segura, Lino
AU  - Sanchez-Segura L
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Km. 9.6 Libramiento 
      Norte Carr. Irapuato-Leon 36824 Irapuato Gto., Mexico.
FAU - Cordero-Martinez, Emmanuel
AU  - Cordero-Martinez E
AD  - Laboratorio de Ciencias Agrogenomicas, de la Escuela Nacional de Estudios 
      Superiores Unidad Leon, Universidad Nacional Autonoma de Mexico, Blv. UNAM 2011, 
      Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Leon 
      Gto., Mexico.
FAU - Vega-Arreguin, Julio C
AU  - Vega-Arreguin JC
AD  - Laboratorio de Ciencias Agrogenomicas, de la Escuela Nacional de Estudios 
      Superiores Unidad Leon, Universidad Nacional Autonoma de Mexico, Blv. UNAM 2011, 
      Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Leon 
      Gto., Mexico.
FAU - Ruiz-Herrera, Jose
AU  - Ruiz-Herrera J
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Km. 9.6 Libramiento 
      Norte Carr. Irapuato-Leon 36824 Irapuato Gto., Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Yeast Res
JT  - FEMS yeast research
JID - 101085384
RN  - 0 (Culture Media)
RN  - 0 (Reactive Oxygen Species)
RN  - 9100L32L2N (Metformin)
RN  - IT942ZTH98 (Curcumin)
RN  - Sporisorium reilianum
RN  - Ustilago maydis
SB  - IM
MH  - Basidiomycota/*cytology/drug effects
MH  - *Cell Death
MH  - Culture Media
MH  - Curcumin/*pharmacology
MH  - Metformin/*pharmacology
MH  - Microbial Viability
MH  - Reactive Oxygen Species
MH  - Saccharomyces cerevisiae
MH  - Yarrowia
OTO - NOTNLM
OT  - Sporisorium reilianum
OT  - Ustilago maydis
OT  - cell death
OT  - curcumin
OT  - lifespan
OT  - metformin
EDAT- 2020/09/19 06:00
MHDA- 2021/09/25 06:00
CRDT- 2020/09/18 12:12
PHST- 2019/12/28 00:00 [received]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/09/19 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
PHST- 2020/09/18 12:12 [entrez]
AID - 5908381 [pii]
AID - 10.1093/femsyr/foaa051 [doi]
PST - ppublish
SO  - FEMS Yeast Res. 2020 Oct 15;20(7):foaa051. doi: 10.1093/femsyr/foaa051.