PMID- 3295136
OWN - NLM
STAT- MEDLINE
DCOM- 19870713
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 57
IP  - 4
DP  - 1987 Apr
TI  - Cell size, projection path, and localization of estrogen-sensitive neurons in the 
      rat ventromedial hypothalamus.
PG  - 1148-59
AB  - Antidromic action potentials were recorded in and around the ventromedial nucleus 
      of the hypothalamus (VMN) of the ovariectomized female rat following stimulation 
      of the central gray matter of the mesencephalon (CG). The animals had either the 
      lateral or caudal trajectory of the VMN efferent disrupted by a small knife cut. 
      Changes in the polarity, peak-to-peak amplitude, and the duration of the initial 
      positive deflection of the antidromic action potentials were recorded for each 
      response as the recording electrode was advanced at 10-micron intervals from the 
      point of detection of the potential until its deterioration. Seventy-four 
      recordings in which potentials exceeded 2 mV at the maxima were subjected to 
      further analysis. The mean spatial spread of the extracellular antidromic action 
      potentials, which was measured by moving the recording electrode at 10-micron 
      intervals, was significantly larger in responses in 17 animals bearing the 
      posterior knife cut (n = 41) than those in 15 animals with the lateral knife cut 
      (n = 33). Mean peak-to-peak amplitude was also significantly greater in responses 
      from the posterior-cut animals than in those from the lateral-cut ones. In the 
      former, action potentials were completed with a shorter time lapse than the 
      latter. In the posterior-cut animals, estrogen treatment of the ovariectomized 
      rats significantly lowered antidromic activation thresholds and shortened 
      absolute refractory periods for CG stimulation. Estrogen had no detectable effect 
      on the excitability of cells that survived the lateral cut. Histological analyses 
      located antidromically driven cells in the posterior-cut animals in the rostral 
      extremity of the VMN and the adjacent retrochiasmatic area; those in the 
      lateral-cut animals were scattered in and around the VMN, caudad to the former. 
      These results suggest that rostral VMN neurons with large soma and laterally 
      projecting axons are responsible for estrogen-dependent autonomic, 
      neuroendocrine, and behavioral functions, such as reproduction and feeding.
FAU - Sakuma, Y
AU  - Sakuma Y
FAU - Akaishi, T
AU  - Akaishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Efferent Pathways/physiology
MH  - Electric Stimulation
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Neurons/drug effects/*physiology
MH  - Ovariectomy
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Stereotaxic Techniques
MH  - Ventromedial Hypothalamic Nucleus/drug effects/*physiology
EDAT- 1987/04/01 00:00
MHDA- 1987/04/01 00:01
CRDT- 1987/04/01 00:00
PHST- 1987/04/01 00:00 [pubmed]
PHST- 1987/04/01 00:01 [medline]
PHST- 1987/04/01 00:00 [entrez]
AID - 10.1152/jn.1987.57.4.1148 [doi]
PST - ppublish
SO  - J Neurophysiol. 1987 Apr;57(4):1148-59. doi: 10.1152/jn.1987.57.4.1148.