PMID- 32951582
OWN - NLM
STAT- MEDLINE
DCOM- 20211004
LR  - 20211004
IS  - 1875-5992 (Electronic)
IS  - 1871-5206 (Linking)
VI  - 21
IP  - 6
DP  - 2021
TI  - Comparative Analysis of the Rabbit Endothelial Progenitor Cells from Bone Marrow 
      and Peripheral Blood Treated with Selenium Nanoparticles.
PG  - 803-808
LID - 10.2174/1871520620666200918112552 [doi]
AB  - BACKGROUND: Selenium Nanoparticles (Se-NPs) are known for their antioxidant and 
      anti-inflammatory activities, which are effective in preventing oxidative damage 
      and improving physiological processes. OBJECTIVES: This study aimed at 
      investigating the effects of biosynthesized Se-NPs on bone marrow-derived 
      Endothelial Progenitor Cells (bone marrow-derived EPCs) and blood-derived 
      endothelial progenitor cells (blood-derived EPCs) isolated from rabbits in vitro. 
      METHODS: The cultured EPCs incubated with biosynthesized Se-NPs at the 
      concentrations of 0.19, 0.38, 0.76, 1.71, 3.42, 7.03, 14.25, 28.50, 57, 114, and 
      228mug/ml for 48h. After screening the proliferative potential of the Se-NPs by 
      the MTT assay, the best concentrations were selected for Real-Time quantitative 
      Polymerase Chain Reaction (RT-qPCR). Real-time quantification of Vascular Cell 
      Adhesion Molecule 1 (VCAM-1), lectin-like oxidized Low-Density Lipoprotein (LDL) 
      receptor-1 (LOX-1), endothelial Nitric Oxide Synthase (eNOS), and Monocyte 
      Chemoattractant Protein-1 (MCP-1) gene expressions were analyzed by normalizing 
      with Glyceraldehyde- 3-Phosphate Dehydrogenase (GAPDH) as an endogenous reference 
      gene. RESULTS: Blood-derived EPCs and bone marrow-derived EPCs showed 
      morphological differences before treatment in vitro. Se-NPs treated EPCs 
      indicated a significant dose-dependent proliferative activity (p<0.01). In 
      general, the expression levels of VCAM-1, LOX-1, and MCP-1 mRNA were 
      significantly decreased (p<0.01), whereas that of the eNOS expression was 
      significantly increased at the concentrations of 7.3 and 14.25mug/ml (p<0.01). 
      Although the expressions of MCP-1, LOX-1, and eNOS mRNA were decreased at certain 
      concentrations of Se-NPs (p<0.01 and p<0.05, respectively) in the treated bone 
      marrow-derived EPCs, no significant differences were observed in the VCAM-1 mRNA 
      expression levels in bone marrow-derived EPCs compared with the control group 
      (p>0.05). CONCLUSION: This was the first report to demonstrate the effects of 
      Se-NPs on proliferative, anti-oxidative, and anti-inflammatory activities for 
      bone marrow-derived EPCs and blood-derived EPCs. Our findings suggested that 
      Se-NPs could be considered as an effective agent that may ameliorate vascular 
      problems.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Shoeibi, Sara
AU  - Shoeibi S
AD  - Department of Medical Biotechnology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Anticancer Agents Med Chem
JT  - Anti-cancer agents in medicinal chemistry
JID - 101265649
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*chemistry/pharmacology
MH  - Antioxidants/*chemistry/pharmacology
MH  - Blood Cells/cytology
MH  - Bone Marrow
MH  - Chemokine CCL2/genetics/metabolism
MH  - Endothelial Progenitor Cells/*drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/metabolism
MH  - Humans
MH  - Nanomedicine
MH  - Nanoparticles/*chemistry
MH  - Nitric Oxide Synthase Type III/genetics/metabolism
MH  - Rabbits
MH  - Scavenger Receptors, Class E/genetics/metabolism
MH  - Selenium/*chemistry/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
OTO - NOTNLM
OT  - EPCs
OT  - Se-NPs
OT  - anti-oxidative
OT  - eNOS
OT  - inflammation
OT  - proliferation
EDAT- 2020/09/22 06:00
MHDA- 2021/10/05 06:00
CRDT- 2020/09/21 05:31
PHST- 2020/03/09 00:00 [received]
PHST- 2020/07/05 00:00 [revised]
PHST- 2020/08/08 00:00 [accepted]
PHST- 2020/09/22 06:00 [pubmed]
PHST- 2021/10/05 06:00 [medline]
PHST- 2020/09/21 05:31 [entrez]
AID - ACAMC-EPUB-110065 [pii]
AID - 10.2174/1871520620666200918112552 [doi]
PST - ppublish
SO  - Anticancer Agents Med Chem. 2021;21(6):803-808. doi: 
      10.2174/1871520620666200918112552.