PMID- 32952497
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220417
IS  - 1654-661X (Print)
IS  - 1654-661X (Electronic)
IS  - 1654-661X (Linking)
VI  - 64
DP  - 2020
TI  - Effect of dietary oils from various sources on carbohydrate and fat metabolism in 
      mice.
LID - 10.29219/fnr.v64.4287 [doi]
AB  - BACKGROUND: Dietary oils differ in their fatty acid composition and the presence 
      of additional microcomponents (antioxidants, etc.). These differences are thought 
      to invoke different biochemical pathways, thus affecting fats and carbohydrates 
      metabolism differently. Olive oil (OO) and soybean oil (SO) are common vegetable 
      oils in the local cuisine. Peanuts oils of local varieties are viewed as 
      potential sources of dietary vegetable oils, especially in the food industry. 
      OBJECTIVE: We examined the effect of four different dietary vegetable oils on 
      carbohydrate and lipid metabolism in mice. The selected oils were OO, high in 
      oleic acid, extracted from cultivated high oleic acid peanut (C-PO), regular 
      peanut oil (PO), and SO. DESIGN: In this study, 32 male C57BL/6J mice were 
      randomly divided into four groups (n = 8 in each group) and were fed with four 
      different diets enriched with 4% (w/w) dietary vegetable oils (OO, C-PO, PO, or 
      SO). After 10 weeks, the mice were sacrificed. Western blot was used to examine 
      proteins such as phospho-AMP-activated protein kinase (p-AMPK), ace-tyl-CoA 
      carboxylase (ACC), cluster of differentiation 36 (CD36), and Sirtuin 1 (SIRT1), 
      whereas real-time polymerase chain reaction (PCR) was used to examine the 
      expression of sterol regulatory element-binding protein-1c (SREBP-1C), fatty acid 
      synthase (FAS), glucose-6-phosphatase (G6Pase), and CD36 transcripts. RESULTS: In 
      mice-fed SO, lipid accumulation was predominately in adipose tissue, accompanied 
      a tendency decrease in insulin sensitivity. Mice-fed OO had lower plasma 
      triglycerides (TG) and increased hepatic CD36 gene expression. The C-PO group 
      presented lower messenger RNA (mRNA) levels in the liver for all examined genes: 
      SREBP-1c, FAS, G6Pase, and CD36. There were no significant differences in weight 
      gain, plasma cholesterol and high-density lipoprotein (HDL) cholesterol levels, 
      hepatic ACC, SIRT1, AMPK, and CD36 protein levels or in liver function among the 
      diets. DISCUSSION: It seems that as long as fat is consumed in moderation, oil 
      types may play a lesser role in the metabolism of healthy individuals. 
      CONCLUSION: This finding has the potential to increase flexibility in choosing 
      oil types for consumption.
CI  - (c) 2020 Anna Altberg et al.
FAU - Altberg, Anna
AU  - Altberg A
AD  - Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of 
      Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 
      Israel.
FAU - Hovav, Ran
AU  - Hovav R
AD  - Department of Field and Vegetable Crops, Plant Sciences Institute, ARO (Volcani 
      Center), Bet Dagan, Israel.
FAU - Chapnik, Nava
AU  - Chapnik N
AD  - Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of 
      Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 
      Israel.
FAU - Madar, Zecharia
AU  - Madar Z
AD  - Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of 
      Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 
      Israel.
LA  - eng
PT  - Journal Article
DEP - 20200813
PL  - Sweden
TA  - Food Nutr Res
JT  - Food & nutrition research
JID - 101488795
PMC - PMC7478119
OTO - NOTNLM
OT  - D7 oil
OT  - gene expression
OT  - oleic acid
OT  - peanut oil
OT  - soybean oil
OT  - triglycerides
COIS- The authors declare no conflicts of interest. This research received no external 
      funding.
EDAT- 2020/09/22 06:00
MHDA- 2020/09/22 06:01
PMCR- 2020/08/13
CRDT- 2020/09/21 06:06
PHST- 2020/01/15 00:00 [received]
PHST- 2020/06/04 00:00 [revised]
PHST- 2020/06/10 00:00 [accepted]
PHST- 2020/09/21 06:06 [entrez]
PHST- 2020/09/22 06:00 [pubmed]
PHST- 2020/09/22 06:01 [medline]
PHST- 2020/08/13 00:00 [pmc-release]
AID - 4287 [pii]
AID - 10.29219/fnr.v64.4287 [doi]
PST - epublish
SO  - Food Nutr Res. 2020 Aug 13;64. doi: 10.29219/fnr.v64.4287. eCollection 2020.