PMID- 32955526
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220417
IS  - 1016-3190 (Print)
IS  - 2223-8956 (Electronic)
VI  - 32
IP  - 3
DP  - 2020 Jul-Sep
TI  - The deduced probable HLA-C*03:187-associated human leukocyte antigen haplotype 
      (A*24:02-B*35:01-C*03:187-DRB1*11:01) revealed in Taiwanese unrelated 
      hematopoietic bone marrow stem cell donors.
PG  - 254-257
LID - 10.4103/tcmj.tcmj_106_19 [doi]
AB  - OBJECTIVE: HLA-C*03:187 is a rare frequency allele in the human leukocyte antigen 
      (HLA)-C locus. The purpose of this investigation is to indicate the ethnicity of 
      C*03:187 and its deduced plausible HLA haplotype in association in Taiwanese 
      unrelated bone marrow stem cell donors. MATERIALS AND METHODS: A DNA 
      sequence-based typing procedure was used to verify the rare frequency allele 
      C*03:187. Employing group-specific primer sets' polymerase chain reaction was 
      carried out to amplify exons 2 and 3 of HLA-A locus, HLA-B locus, exon 1 to exon 
      7 of the HLA-C locus, and exon 2 of the HLA-DRB1 locus. The amplified gene 
      products were sequenced employing the BigDye((R)) Terminator Cycle Sequencing Ready 
      Reaction kits in both directions according to the manufacturer's instructions. 
      RESULTS: The DNA sequence of C*03:187 is identical to C*03:03:01:01 from exon 1 
      to exon 7, except for codon 152 of exon 3 where GAG of C*03:03:01:01 is replaced 
      by GTG in C*03:187. The nucleotide replacement causes one amino acid change to 
      the protein sequence of C*03:03:01:01 at position 152 where glutamic acid (E) is 
      changed to a valine (V) in C*03:187. The deduced plausible HLA haplotype in 
      association with C*03:187 in Taiwanese is as A*24:02-B*35:01-C*03:187-DRB1*11:01. 
      CONCLUSION: The data on the deduced plausible HLA haplotype in association with 
      the low-frequency C*03:187 allele that we described in this report are valuable 
      for immunogenetics laboratories for reference purposes. In addition, they can be 
      utilized by search coordinators in hematopoietic stem cell transplant programs to 
      determine a strategy for locating compatible donors in unrelated bone marrow 
      donor registries for a patient with this unusual HLA allele.
CI  - Copyright: (c) 2019 Tzu Chi Medical Journal.
FAU - Yang, Kuo-Liang
AU  - Yang KL
AD  - Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank and Buddhist Tzu Chi Bone 
      Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi 
      Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
AD  - Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, 
      Taiwan.
FAU - Lin, Py-Yu
AU  - Lin PY
AD  - Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank and Buddhist Tzu Chi Bone 
      Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi 
      Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20190930
PL  - China (Republic : 1949- )
TA  - Ci Ji Yi Xue Za Zhi
JT  - Ci ji yi xue za zhi = Tzu-chi medical journal
JID - 9514171
PMC - PMC7485676
OTO - NOTNLM
OT  - HLA-C*03:187
OT  - Haplotype
OT  - Human leukocyte antigen
OT  - Sequence-based typing
OT  - Taiwanese
COIS- There are no conflicts of interest.
EDAT- 2020/09/22 06:00
MHDA- 2020/09/22 06:01
PMCR- 2019/09/30
CRDT- 2020/09/21 12:12
PHST- 2019/05/09 00:00 [received]
PHST- 2019/05/13 00:00 [revised]
PHST- 2019/07/18 00:00 [accepted]
PHST- 2020/09/21 12:12 [entrez]
PHST- 2020/09/22 06:00 [pubmed]
PHST- 2020/09/22 06:01 [medline]
PHST- 2019/09/30 00:00 [pmc-release]
AID - TCMJ-32-254 [pii]
AID - 10.4103/tcmj.tcmj_106_19 [doi]
PST - epublish
SO  - Ci Ji Yi Xue Za Zhi. 2019 Sep 30;32(3):254-257. doi: 10.4103/tcmj.tcmj_106_19. 
      eCollection 2020 Jul-Sep.