PMID- 32958127
OWN - NLM
STAT- MEDLINE
DCOM- 20201110
LR  - 20201110
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 36
IP  - 8
DP  - 2020 Aug
TI  - [Bioinformatic analysis of Eftud2 enhancing mouse macrophage function].
PG  - 704-711
AB  - Objective To elucidate the mechanisms by which elongation factor Tu GTP binding 
      domain containing 2 (Eftud2) enhances the immune function of murine macrophages 
      by bioinformatics analysis. Methods The bone marrow-derived macrophages (BMDMs) 
      of Eftud2 myeloid cell-specific knockout (MKO) mice (n=10) and wild-type (WT) 
      littermates (n=10) were collected and stimulated by lipopolysaccharide (LPS) (100 
      ng/mL) for 2 hours. Bioinformatics analysis was conducted to examine the 
      differences in gene expression and mRNA transcription levels. The the differences 
      in gene expression and alternative splicing of mRNA transcription in BMDMs were 
      analyzed by DEGseq and rMATS, respectively. The signaling pathways affected were 
      clarified by Kyoto Encyclopedia of Genes and Genomes (KEGG) classification and 
      enrichment methods. Results Compared with WT counterparts, the expression levels 
      of IL-6, IL-1beta, TNF-alpha, and the genes related to immune response in MKO BMDMs were 
      down-regulated following LPS stimulation. KEGG pathway analysis showed that the 
      differently expressed genes in BMDMs and alternative splicing mainly affected the 
      signal transduction and immune system-related metabolic pathways, and had a 
      strong correlation with PI3K-AKT signaling pathway. The difference in alternative 
      splicing also existed in ubiquitination and endocytosis. Compared with WT 
      counterparts, there were 232 differences in alternative splicing in MKO BMDMs, 
      among which 125 were skipping exons, accounting for the largest proportion. In 
      addition, the analysis of alternative splicing differences also confirmed the 
      previous experimental results, that is, Eftud2 could participate in the 
      activation of inflammatory signaling pathways by enhancing the alternative 
      splicing of key molecules such as MyD88 in TLR4-NF-kappaB signaling pathway, thereby 
      augmenting the function of macrophages. Conclusion Eftud2 can promote the release 
      of inflammatory cytokines in BMDMs by regulating gene expression and alternative 
      splicing, and consequently enhance the immune function of macrophages.
FAU - Sun, Ying
AU  - Sun Y
AD  - Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical 
      University, Hohhot 010058; Institute of Pharmacology and Toxicology, Academy of 
      Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
FAU - Yang, Peng
AU  - Yang P
AD  - Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical 
      University, Hohhot 010058; Institute of Pharmacology and Toxicology, Academy of 
      Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
FAU - Lyu, Zhonglin
AU  - Lyu Z
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, 
      Academy of Military Sciences, Beijing 100850; Department of Hematology, Second 
      Medical Center, PLA General Hospital, Beijing 100039, China.
FAU - Zheng, Yuanqiang
AU  - Zheng Y
AD  - Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical 
      University, Hohhot 010058, China.
FAU - Shi, Yanchun
AU  - Shi Y
AD  - Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical 
      University, Hohhot 010058, China. *Corresponding authors, E-mail: 
      ycshi5388@163.com.
FAU - Chen, Guojiang
AU  - Chen G
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, 
      Academy of Military Sciences, Beijing 100850, China. *Corresponding authors, 
      E-mail: jyk62033@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - *Computational Biology
MH  - Cytokines/genetics/immunology
MH  - *Gene Expression/genetics/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - *Macrophages/immunology
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
EDAT- 2020/09/23 06:00
MHDA- 2020/11/11 06:00
CRDT- 2020/09/22 05:31
PHST- 2020/09/22 05:31 [entrez]
PHST- 2020/09/23 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Aug;36(8):704-711.