PMID- 32959209
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1936-0568 (Electronic)
IS  - 1936-055X (Print)
IS  - 1936-055X (Linking)
VI  - 15
IP  - 2
DP  - 2021 Jun
TI  - Expression of Mucins in Salivary Gland Mucoepidermoid Carcinoma.
PG  - 491-502
LID - 10.1007/s12105-020-01226-z [doi]
AB  - Mucoepidermoid carcinoma (MEC) is the most common malignant salivary gland tumour 
      in both adults and children. Histological grading of MEC is subjective, but plays 
      an important role in predicting patient prognosis. Epithelial mucin (MUC) status 
      may aid in establishing a more accurate grade. This study aimed to investigate 
      the expression of various mucins (MUC1, MUC2, MUC4 and MUC5AC) in MECs to 
      determine a possible correlation with tumour grade. Fifteen cases of each tumour 
      grade (low-, intermediate-, and high-grade) were retrieved from the pathology 
      archives of the Department of Oral Pathology and Oral Biology at the University 
      of Pretoria. The patients included 23 men and 22 women, and ranged from 13 to 
      85 years (mean 49.8 years). Sections from formalin-fixed paraffin-embedded (FFPE) 
      tissue were used for fluorescence in situ hybridization (FISH) for MAML2 
      rearrangements and MUC immunohistochemical analysis. The percentage 
      immunohistochemical expression of the neoplastic mucous cells was evaluated 
      first, followed by the overall percentage expression of all tumour cells. The 
      results indicated that MUC1 overexpression may be a reliable marker of high-grade 
      MECs, whereas MUC4 overexpression may be more indicative of low-grade tumours. 
      MUC5AC expression was considered an unreliable marker in determining grade. MUC2 
      was only expressed in a single case of MEC and may be considered a useful marker 
      to exclude MEC as a diagnostic possibility. This study demonstrates that MECs 
      show an altered MUC expression pattern that can be used for diagnostic purposes 
      and to aid in establishing a more accurate tumour grade.
FAU - Robinson, Liam
AU  - Robinson L
AUID- ORCID: 0000-0002-0549-7824
AD  - Department of Oral Pathology and Oral Biology, School of Dentistry, Faculty of 
      Health Sciences, University of Pretoria, Pretoria, South Africa.
FAU - van Heerden, Marlene B
AU  - van Heerden MB
AUID- ORCID: 0000-0002-3710-8352
AD  - Department of Oral Pathology and Oral Biology, School of Dentistry, Faculty of 
      Health Sciences, University of Pretoria, Pretoria, South Africa.
FAU - Ker-Fox, Jason G
AU  - Ker-Fox JG
AUID- ORCID: 0000-0002-4660-7612
AD  - Department of Financial Management, Faculty of Economic and Management Sciences, 
      University of Pretoria, Pretoria, South Africa.
FAU - Hunter, Keith D
AU  - Hunter KD
AUID- ORCID: 0000-0002-7873-0877
AD  - Department of Oral Pathology and Oral Biology, School of Dentistry, Faculty of 
      Health Sciences, University of Pretoria, Pretoria, South Africa.
AD  - Academic Unit of Oral and Maxillofacial Medicine and Pathology, School of 
      Clinical Dentistry, University of Sheffield, Sheffield, UK.
FAU - van Heerden, Willie F P
AU  - van Heerden WFP
AUID- ORCID: 0000-0003-2494-667X
AD  - Department of Oral Pathology and Oral Biology, School of Dentistry, Faculty of 
      Health Sciences, University of Pretoria, Pretoria, South Africa. 
      willie.vanheerden@up.ac.za.
LA  - eng
PT  - Journal Article
DEP - 20200921
PL  - United States
TA  - Head Neck Pathol
JT  - Head and neck pathology
JID - 101304010
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Mucins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis/metabolism
MH  - Carcinoma, Mucoepidermoid/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mucins/analysis/*biosynthesis
MH  - Salivary Gland Neoplasms/metabolism/*pathology
MH  - Young Adult
PMC - PMC8134608
OTO - NOTNLM
OT  - Epithelial mucin expression
OT  - FISH
OT  - Histological grade
OT  - Immunohistochemistry
OT  - Mucoepidermoid carcinoma
OT  - Salivary gland carcinoma
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/09/23 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/09/21
CRDT- 2020/09/22 05:44
PHST- 2020/07/31 00:00 [received]
PHST- 2020/09/11 00:00 [accepted]
PHST- 2020/09/23 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/09/22 05:44 [entrez]
PHST- 2021/09/21 00:00 [pmc-release]
AID - 10.1007/s12105-020-01226-z [pii]
AID - 1226 [pii]
AID - 10.1007/s12105-020-01226-z [doi]
PST - ppublish
SO  - Head Neck Pathol. 2021 Jun;15(2):491-502. doi: 10.1007/s12105-020-01226-z. Epub 
      2020 Sep 21.