PMID- 32959549
OWN - NLM
STAT- MEDLINE
DCOM- 20201112
LR  - 20201112
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 45
IP  - 9
DP  - 2020 Sep 25
TI  - [Effects of electroacupuncture on cognitive function and neuronal autophagy in 
      rats with D-galactose induced Alzheimer's disease].
PG  - 689-95
LID - 10.13702/j.1000-0607.190854 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture at "Baihui"(GV20) and 
      "Shenshu"(BL23) on the expression of autophagy-related proteins in the 
      hippocampus of rats with Alzheimer's disease (AD)，so as to explore its underlying 
      mechanisms on improvement of AD. METHODS: Forty-eight male SD rats were randomly 
      divided into blank control group, model group, electroacupuncture group and sham 
      electroacupuncture group, with 12 rats in each group. The AD rat model was 
      establish by intraperitoneal injection of D-galactose for 6 weeks. Rats in the 
      electroacupuncture group received electroacupuncture (50 Hz, 1 mA)at GV20 and 
      BL23 for 20 min each time after daily intraperitoneal injection. Rats in the sham 
      electroacupuncture group received acupuncture at the local skin of GV20 and BL23 
      without electricity. After the intervention, Morris water maze and open field 
      test were used to evaluate the learning and cognitive ability of rats in each 
      group. The transmission electron microscope was used to observe the numerical 
      density of synaptic in hippocampus, and the immunohistochemical staining was used 
      to observe the paired helical filament protein-1 (PHF-1) in the hippocampus. 
      Western blot was used to detected the expression of autophagy-related proteins 
      phosphoinositide-3-kinase (PI3K), protein kinase B (AKT), phosphorylated AKT 
      (p-AKT), mammalian target of rapamycin (mTOR) in the hippocampus. RESULTS: 
      Compared with the blank control group, the escape latency of the rats in the 
      model group increased from day 2 to day 5 (P<0.01), and the ratio of the time 
      through the quadrant of the original platform reduced （P<0.01）, in the open field 
      test the distance of exercise, the number of uprights and the rate of exercise 
      time in the central area decreased (P<0.01), meanwhile the density of hippocampus 
      synapses decreased (P<0.01), the positive expression of PHF-1 and the relative 
      expression of PI3K, AKT, p-AKT, and mTOR all increased (P<0.01). Compared with 
      the model group, the escape latency of rats in the electroacupuncture group was 
      shortened from day 2 to day 5 (P<0.01), and the ratio of the time through the 
      quadrant of the original platform meanwhile, the distance of the open field test, 
      the number of uprights, and the rate of central area exercise time up-regulated 
      (P<0.01), the numerical density of hippocampus synatic increased (P<0.01), the 
      positive expression of PHF-1 and the relative expression of PI3K, AKT, p-AKT, and 
      mTOR all down-regulated (P<0.01). Compared with the model group, the expression 
      of PI3K in the sham electroacupuncture group decreased (P<0.05). CONCLUSION: 
      Electroacupuncture can improve learning and memory and cognitive impairment in AD 
      rats, which may be associated with its effects in regulation of hippocampal 
      autophagy and removal of neurofibrillary tangles by suppressing PI3K/AKT/mTOR 
      signaling pathway.
FAU - Zheng, Qing
AU  - Zheng Q
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Kong, Li-Hong
AU  - Kong LH
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Yu, Chao-Chao
AU  - Yu CC
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - He, Rui-Yang
AU  - He RY
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Wang, Xue-Song
AU  - Wang XS
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Ma, Ran
AU  - Ma R
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Chen, Yun-Yun
AU  - Chen YY
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
FAU - Wang, Yue-Shen
AU  - Wang YS
AD  - College of Acupuncture-moxibustion and Massage, Hubei University of Chinese 
      Medicine, Wuhan 430061, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - *Alzheimer Disease/genetics/therapy
MH  - Animals
MH  - *Autophagy
MH  - Cognition
MH  - *Electroacupuncture
MH  - Galactose
MH  - Hippocampus
MH  - Male
MH  - Phosphatidylinositol 3-Kinases
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Autophagy
OT  - Cognitive function
OT  - Electroacupuncture
OT  - PI3K/AKT/mTOR signaling pathway
EDAT- 2020/09/23 06:00
MHDA- 2020/11/13 06:00
CRDT- 2020/09/22 05:48
PHST- 2020/09/22 05:48 [entrez]
PHST- 2020/09/23 06:00 [pubmed]
PHST- 2020/11/13 06:00 [medline]
AID - 10.13702/j.1000-0607.190854 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2020 Sep 25;45(9):689-95. doi: 10.13702/j.1000-0607.190854.