PMID- 32961672
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201023
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 10
IP  - 9
DP  - 2020 Sep 19
TI  - Evaluation of the NLRP3 Inflammasome Activating Effects of a Large Panel of 
      TiO(2) Nanomaterials in Macrophages.
LID - 10.3390/nano10091876 [doi]
LID - 1876
AB  - TiO(2) nanomaterials are among the most commonly produced and used engineered 
      nanomaterials (NMs) in the world. There is controversy regarding their ability to 
      induce inflammation-mediated lung injuries following inhalation exposure. 
      Activation of the NACHT, LRR and PYD domains-containing protein 3 (NALP3) 
      inflammasome and subsequent release of the cytokine interleukin (IL)-1beta in 
      pulmonary macrophages has been postulated as an essential pathway for the 
      inflammatory and associated tissue-remodeling effects of toxic particles. Our 
      study aim was to determine and rank the IL-1beta activating properties of TiO(2) NMs 
      by comparing a large panel of different samples against each other as well as 
      against fine TiO(2), synthetic amorphous silica and crystalline silica (DQ12 
      quartz). Effects were evaluated in primary bone marrow derived macrophages 
      (BMDMs) from NALP3-deficient and proficient mice as well as in the rat alveolar 
      macrophage cell line NR8383. Our results show that specific TiO(2) NMs can 
      activate the inflammasome in macrophages albeit with a markedly lower potency 
      than amorphous SiO(2) and quartz. The heterogeneity in IL-1beta release observed in 
      our study among 19 different TiO(2) NMs underscores the relevance of case-by-case 
      evaluation of nanomaterials of similar chemical composition. Our findings also 
      further promote the NR8383 cell line as a promising in vitro tool for the 
      assessment of the inflammatory and inflammasome activating properties of NMs.
FAU - Kolling, Julia
AU  - Kolling J
AD  - IUF-Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 
      40225 DE Dusseldorf, Germany.
FAU - Tigges, Jonas
AU  - Tigges J
AD  - IUF-Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 
      40225 DE Dusseldorf, Germany.
AD  - Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937 
      DE Munich, Germany.
FAU - Hellack, Bryan
AU  - Hellack B
AD  - Institute of Energy and Environmental Technology e.V. (IUTA), Bliersheimer Str. 
      60, 47229 Duisburg, Germany.
AD  - UBA-German Environment Agency, Paul-Ehrlich-Str. 29, 63225 Langen, Germany.
FAU - Albrecht, Catrin
AU  - Albrecht C
AD  - IUF-Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 
      40225 DE Dusseldorf, Germany.
AD  - State Office for Consumer Protection Saxony-Anhalt, 39576 Stendal, Germany.
FAU - Schins, Roel P F
AU  - Schins RPF
AD  - IUF-Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 
      40225 DE Dusseldorf, Germany.
LA  - eng
GR  - FP7-NMP-2013-LARGE-7/Seventh Framework Programme/
PT  - Journal Article
DEP - 20200919
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7558067
OTO - NOTNLM
OT  - NALP3
OT  - NR8383
OT  - bone marrow derived macrophages
OT  - interleukin-1beta
OT  - nanomaterials
OT  - titanium dioxide
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/09/24 06:00
MHDA- 2020/09/24 06:01
PMCR- 2020/09/19
CRDT- 2020/09/23 01:01
PHST- 2020/07/16 00:00 [received]
PHST- 2020/09/10 00:00 [revised]
PHST- 2020/09/15 00:00 [accepted]
PHST- 2020/09/23 01:01 [entrez]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2020/09/24 06:01 [medline]
PHST- 2020/09/19 00:00 [pmc-release]
AID - nano10091876 [pii]
AID - nanomaterials-10-01876 [pii]
AID - 10.3390/nano10091876 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2020 Sep 19;10(9):1876. doi: 10.3390/nano10091876.