PMID- 32961914
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201023
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 10
IP  - 9
DP  - 2020 Sep 18
TI  - Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce 
      Proinflammatory Effects in Human Lung Fibroblasts.
LID - 10.3390/nano10091868 [doi]
LID - 1868
AB  - Silver nanoparticles (AgNPs) are commonly used in commercial and medical 
      applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) 
      changes and inflammatory responses. Fibroblasts are key players in remodeling 
      processes and major producers of the ECM. The aims of this study were to explore 
      the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung 
      slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and 
      immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs 
      with different sizes, 10 nm and 75 nm, at concentrations 2 microg/mL and 10 mug/mL. 
      Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed 
      in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced 
      metabolic activities at 10 mug/mL in both PCLS and HFL-1 after 48 h. AgNPs 
      significantly increased procollagen I synthesis and release of IL-8, 
      prostaglandin E(2), RANTES and eotaxin, whereas reduced IL-6 release was observed 
      in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell 
      viability ex vivo and in vitro with altered procollagen and proinflammatory 
      cytokine secretion in fibroblasts over time. Hence, careful characterizations of 
      AgNPs are of importance, and future studies should include timepoints beyond 24 
      h.
FAU - Lofdahl, Anna
AU  - Lofdahl A
AD  - Unit of Lung Biology, Department of Experimental Medical Sciences, Lund 
      University, SE-221 00 Lund, Sweden.
FAU - Jern, Andreas
AU  - Jern A
AD  - Unit of Lung Biology, Department of Experimental Medical Sciences, Lund 
      University, SE-221 00 Lund, Sweden.
FAU - Flyman, Samuel
AU  - Flyman S
AD  - Unit of Lung Biology, Department of Experimental Medical Sciences, Lund 
      University, SE-221 00 Lund, Sweden.
FAU - Karedal, Monica
AU  - Karedal M
AD  - Division of Occupational and Environmental Medicine, Lund University, SE-221 00 
      Lund, Sweden.
FAU - Karlsson, Hanna L
AU  - Karlsson HL
AD  - Unit of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska 
      Institutet, SE-171 76 Stockholm, Sweden.
FAU - Larsson-Callerfelt, Anna-Karin
AU  - Larsson-Callerfelt AK
AUID- ORCID: 0000-0001-7586-3896
AD  - Unit of Lung Biology, Department of Experimental Medical Sciences, Lund 
      University, SE-221 00 Lund, Sweden.
LA  - eng
GR  - VR 3R, 2014-2016/Vetenskapsradet/
GR  - -/Kungliga Fysiografiska Sallskapet i Lund/
GR  - -/Lund University Medical Faculty Foundation/
PT  - Journal Article
DEP - 20200918
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7557856
OTO - NOTNLM
OT  - cytokines
OT  - extracellular matrix
OT  - growth factors
OT  - human lung fibroblasts
OT  - precision-cut lung slices
OT  - procollagen
OT  - silver nanoparticles
OT  - toxicity
COIS- The authors declare no conflict of interest and the funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/09/24 06:00
MHDA- 2020/09/24 06:01
PMCR- 2020/09/18
CRDT- 2020/09/23 01:02
PHST- 2020/08/13 00:00 [received]
PHST- 2020/09/02 00:00 [revised]
PHST- 2020/09/16 00:00 [accepted]
PHST- 2020/09/23 01:02 [entrez]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2020/09/24 06:01 [medline]
PHST- 2020/09/18 00:00 [pmc-release]
AID - nano10091868 [pii]
AID - nanomaterials-10-01868 [pii]
AID - 10.3390/nano10091868 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2020 Sep 18;10(9):1868. doi: 10.3390/nano10091868.