PMID- 32961937
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20210310
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 9
DP  - 2020 Sep 18
TI  - CBF-1 Promotes the Establishment and Maintenance of HIV Latency by Recruiting 
      Polycomb Repressive Complexes, PRC1 and PRC2, at HIV LTR.
LID - 10.3390/v12091040 [doi]
LID - 1040
AB  - The C-promoter binding factor-1 (CBF-1) is a potent and specific inhibitor of the 
      human immunodeficiency virus (HIV)-1 LTR promoter. Here, we demonstrate that the 
      knockdown of endogenous CBF-1 in latently infected primary CD4+ T cells, using 
      specific small hairpin RNAs (shRNA), resulted in the reactivation of latent HIV 
      proviruses. Chromatin immunoprecipitation (ChIP) assays using latently infected 
      primary T cells and Jurkat T-cell lines demonstrated that CBF-1 induces the 
      establishment and maintenance of HIV latency by recruiting polycomb group 
      (PcG/PRC) corepressor complexes or polycomb repressive complexes 1 and 2 (PRC1 
      and PRC2). Knockdown of CBF-1 resulted in the dissociation of PRCs corepressor 
      complexes enhancing the recruitment of RNA polymerase II (RNAP II) at HIV LTR. 
      Knockdown of certain components of PRC1 and PRC2 also led to the reactivation of 
      latent proviruses. Similarly, the treatment of latently infected primary CD4+ T 
      cells with the PRC2/EZH2 inhibitor, 3-deazaneplanocin A (DZNep), led to their 
      reactivation.
FAU - Sharma, Adhikarimayum Lakhikumar
AU  - Sharma AL
AUID- ORCID: 0000-0002-9969-8642
AD  - Center for Translational Medicine, Thomas Jefferson University, 1020 Locust 
      Street, Philadelphia, PA 19107, USA.
FAU - Hokello, Joseph
AU  - Hokello J
AUID- ORCID: 0000-0002-6721-5933
AD  - Department of Basic Science, Faculty of Science and Technology, Kampala 
      International University-Western Campus, P.O. Box 71, Bushenyi, Uganda.
FAU - Sonti, Shilpa
AU  - Sonti S
AD  - Center for Translational Medicine, Thomas Jefferson University, 1020 Locust 
      Street, Philadelphia, PA 19107, USA.
FAU - Zicari, Sonia
AU  - Zicari S
AUID- ORCID: 0000-0003-1240-8057
AD  - Section of Intercellular Interactions, Eunice Kennedy-Shriver National Institute 
      of Child Health and Human Development, Bethesda, MD 20892, USA.
AD  - Department of Pediatric Medicine, The Bambino Gesu Children's Hospital, 00165 
      Rome, Italy.
FAU - Sun, Lin
AU  - Sun L
AD  - Division of Infectious Diseases, Department of Medicine, George Washington 
      University, Washington, DC 20037, USA.
FAU - Alqatawni, Aseel
AU  - Alqatawni A
AD  - Center for Translational Medicine, Thomas Jefferson University, 1020 Locust 
      Street, Philadelphia, PA 19107, USA.
FAU - Bukrinsky, Michael
AU  - Bukrinsky M
AUID- ORCID: 0000-0002-8593-0770
AD  - Department of Microbiology, Immunology and Tropical Medicine, George Washington 
      University, Washington, DC 20037, USA.
FAU - Simon, Gary
AU  - Simon G
AD  - Division of Infectious Diseases, Department of Medicine, George Washington 
      University, Washington, DC 20037, USA.
FAU - Chauhan, Ashok
AU  - Chauhan A
AD  - Department of Pathology, Microbiology and Immunology, University of South 
      Carolina School of Medicine, Columbia, SC 29209, USA.
FAU - Daniel, Rene
AU  - Daniel R
AD  - Center for Translational Medicine, Thomas Jefferson University, 1020 Locust 
      Street, Philadelphia, PA 19107, USA.
FAU - Tyagi, Mudit
AU  - Tyagi M
AUID- ORCID: 0000-0003-1493-8051
AD  - Center for Translational Medicine, Thomas Jefferson University, 1020 Locust 
      Street, Philadelphia, PA 19107, USA.
AD  - Division of Infectious Diseases, Department of Medicine, George Washington 
      University, Washington, DC 20037, USA.
LA  - eng
GR  - R01 DA041746/DA/NIDA NIH HHS/United States
GR  - R03 DA033900/DA/NIDA NIH HHS/United States
GR  - R21 DA033924/DA/NIDA NIH HHS/United States
GR  - National Institute on Drug Abuse (NIDA), NIH Grants, 7R01DA041746-02, 
      5R21DA033924-02, 5R03DA033900-02 to MT/NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200918
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Immunoglobulin J Recombination Signal Sequence-Binding Protein)
RN  - 0 (PRC1 protein, human)
RN  - 0 (RBPJ protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cell Line
MH  - Chromatin Immunoprecipitation
MH  - Epigenomics
MH  - Gene Expression Regulation, Viral
MH  - Gene Knockdown Techniques
MH  - HIV Infections/virology
MH  - HIV Long Terminal Repeat
MH  - HIV-1/genetics/*physiology
MH  - Humans
MH  - Immunoglobulin J Recombination Signal Sequence-Binding 
      Protein/genetics/*metabolism
MH  - Promoter Regions, Genetic
MH  - Proviruses/genetics
MH  - RNA Polymerase II/metabolism
MH  - RNA, Small Interfering
MH  - Transcriptome
MH  - Virus Activation
MH  - Virus Latency/genetics/*physiology
PMC - PMC7551090
OTO - NOTNLM
OT  - CBF-1
OT  - HIV-1
OT  - PRC1
OT  - PRC2
OT  - chromatin
OT  - epigenetics
OT  - latency
OT  - transcription
COIS- The authors declare no conflict of interest. The funders had no role in study 
      design, data collection and analysis, decision to publish, or preparation of the 
      manuscript.
EDAT- 2020/09/24 06:00
MHDA- 2021/03/03 06:00
PMCR- 2020/09/01
CRDT- 2020/09/23 01:02
PHST- 2020/08/14 00:00 [received]
PHST- 2020/09/09 00:00 [revised]
PHST- 2020/09/16 00:00 [accepted]
PHST- 2020/09/23 01:02 [entrez]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - v12091040 [pii]
AID - viruses-12-01040 [pii]
AID - 10.3390/v12091040 [doi]
PST - epublish
SO  - Viruses. 2020 Sep 18;12(9):1040. doi: 10.3390/v12091040.