PMID- 32962816
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 225
IP  - 5
DP  - 2020 Sep
TI  - Different roles of intracellular and extracellular reactive oxygen species of 
      neutrophils in type 2 diabetic mice with invasive aspergillosis.
PG  - 151996
LID - S0171-2985(20)30339-9 [pii]
LID - 10.1016/j.imbio.2020.151996 [doi]
AB  - Diabetic patients have an increased risk of invasive aspergillosis (IA), but the 
      mechanism is still unclear. Reactive oxygen species (ROS) produced by neutrophils 
      play a key role in defense against Aspergillus infection. Since diabetes mellitus 
      affects the production of ROS from neutrophils, the purpose of this study is to 
      investigate whether this effect is related to the susceptibility of diabetic mice 
      to IA. C57BL/6 mice were used to establish type 2 diabetes mellitus (T2DM) model, 
      and IA was induced by airway infection with Aspergillus fumigatus. After 
      infection, the fungal load, neutrophil count and ROS content in the lung tissues 
      of T2DM mice were higher than those in the control mice, and the inflammation of 
      the lung tissue was more serious. After being exposed to hyphae in vitro, 
      compared with the control group, neutrophils in T2DM mice had higher apoptosis 
      rate and intracellular ROS content, as well as lower viability, extracellular ROS 
      content and fungicidal ability. In summary, after T2DM mice are infected with A. 
      fumigatus, the reduction of extracellular ROS produced by neutrophils may lead to 
      a decrease in fungicidal ability, while the increase of intracellular ROS is 
      related to neutrophil and lung tissue damage.
CI  - Copyright (c) 2020. Published by Elsevier GmbH.
FAU - Xu, Xianghua
AU  - Xu X
AD  - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital 
      (Changhai Hospital), Naval Medical University, Changhai Road 168, Yangpu, 
      Shanghai 200433, China. Electronic address: ccrysy@sina.com.
FAU - Xia, Chu
AU  - Xia C
AD  - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital 
      (Changhai Hospital), Naval Medical University, Changhai Road 168, Yangpu, 
      Shanghai 200433, China. Electronic address: xia.tianyu@163.com.
FAU - Huang, Yi
AU  - Huang Y
AD  - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital 
      (Changhai Hospital), Naval Medical University, Changhai Road 168, Yangpu, 
      Shanghai 200433, China. Electronic address: huangliur@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200801
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tnf protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.6.3.- (Cybb protein, mouse)
RN  - EC 1.6.3.- (NADPH Oxidase 2)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Aspergillosis/*immunology/microbiology
MH  - *Aspergillus fumigatus
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Cell Survival
MH  - Diabetes Mellitus, Experimental/*immunology/microbiology
MH  - Diabetes Mellitus, Type 2/*immunology/microbiology
MH  - Interleukin-1beta/immunology
MH  - Leukocyte Count
MH  - Lung/immunology
MH  - Lung Diseases/*immunology/microbiology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - NADPH Oxidase 2/immunology
MH  - Neutrophils/*immunology
MH  - Peroxidase/immunology
MH  - Reactive Oxygen Species/*immunology
MH  - Tumor Necrosis Factor-alpha/immunology
OTO - NOTNLM
OT  - Diabetes mellitus
OT  - Invasive aspergillosis
OT  - Neutrophil
OT  - Reactive oxygen species
EDAT- 2020/09/24 06:00
MHDA- 2021/11/05 06:00
CRDT- 2020/09/23 06:03
PHST- 2020/06/24 00:00 [received]
PHST- 2020/07/28 00:00 [revised]
PHST- 2020/07/29 00:00 [accepted]
PHST- 2020/09/23 06:03 [entrez]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
AID - S0171-2985(20)30339-9 [pii]
AID - 10.1016/j.imbio.2020.151996 [doi]
PST - ppublish
SO  - Immunobiology. 2020 Sep;225(5):151996. doi: 10.1016/j.imbio.2020.151996. Epub 
      2020 Aug 1.