PMID- 32964478
OWN - NLM
STAT- MEDLINE
DCOM- 20210105
LR  - 20210105
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 71
IP  - 6
DP  - 2020 Dec
TI  - Efficacy of alcohol-based hand sanitizers against human norovirus using 
      RNase-RT-qPCR with validation by human intestinal enteroid replication.
PG  - 605-610
LID - 10.1111/lam.13393 [doi]
AB  - Successful human norovirus (HuNoV) cultivation in stem cell-derived human 
      intestinal enteroids (HIE) was recently reported. The purpose of this study was 
      to evaluate the anti-HuNoV efficacy of two alcohol-based commercial hand 
      sanitizers and 60% ethanol by suspension assay using RNase-RT-qPCR, with 
      subsequent validation of efficacy by HuNoV cultivation using the HIE model. In 
      suspension, when evaluated by RNase-RT-qPCR, 60% ethanol resulted in less than 
      one log(10) reduction in HuNoV genome equivalent copies (GEC) regardless of 
      contact time (30 or 60s) or soil load. The two commercial products outperformed 
      60% ethanol regardless of contact time or soil load, providing 2.2-3.2 log(10) 
      HuNoV GEC reductions by suspension assay. Product B could not be validated in the 
      HIE model due to cytotoxicity. Following a 60s exposure, viral replication in the 
      HIE model increased 1.9 +/- 0.2 log(10) HuNoV GEC for the neutralization (positive) 
      control and increased 0.9 +/- 0.2 log(10) HuNoV GEC in challenged HIE after 
      treatment with 60% ethanol. No HuNoV replication in HIE was observed after a 60 s 
      exposure to Product A.
CI  - (c) 2020 The Authors. Letters in Applied Microbiology published by John Wiley & 
      Sons Ltd on behalf of Society for Applied Microbiology.
FAU - Escudero-Abarca, B I
AU  - Escudero-Abarca BI
AD  - Department of Food Bioprocessing and Nutrition Sciences, North Carolina State 
      University, Raleigh, NC, USA.
FAU - Goulter, R M
AU  - Goulter RM
AUID- ORCID: 0000-0002-7302-5044
AD  - Department of Food Bioprocessing and Nutrition Sciences, North Carolina State 
      University, Raleigh, NC, USA.
FAU - Arbogast, J W
AU  - Arbogast JW
AD  - GOJO Industries Inc, Akron, OH, USA.
FAU - Leslie, R A
AU  - Leslie RA
AD  - GOJO Industries Inc, Akron, OH, USA.
FAU - Green, K
AU  - Green K
AD  - GOJO Industries Inc, Akron, OH, USA.
FAU - Jaykus, L-A
AU  - Jaykus LA
AD  - Department of Food Bioprocessing and Nutrition Sciences, North Carolina State 
      University, Raleigh, NC, USA.
LA  - eng
GR  - GOJO Industries, Inc./
PT  - Journal Article
DEP - 20201004
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Hand Sanitizers)
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Caliciviridae Infections/*virology
MH  - Drug Evaluation, Preclinical
MH  - Ethanol/*pharmacology
MH  - Hand Sanitizers/*pharmacology
MH  - Humans
MH  - Intestines/*virology
MH  - Norovirus/*drug effects/genetics/growth & development/physiology
MH  - Real-Time Polymerase Chain Reaction/instrumentation
MH  - Ribonucleases/metabolism
MH  - Virus Replication/drug effects
PMC - PMC7756425
OTO - NOTNLM
OT  - RT-qPCR
OT  - disinfection
OT  - food safety
OT  - human intestinal enteroids
OT  - human norovirus
EDAT- 2020/09/24 06:00
MHDA- 2021/01/06 06:00
PMCR- 2020/12/23
CRDT- 2020/09/23 06:40
PHST- 2020/07/13 00:00 [received]
PHST- 2020/09/14 00:00 [revised]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2021/01/06 06:00 [medline]
PHST- 2020/09/23 06:40 [entrez]
PHST- 2020/12/23 00:00 [pmc-release]
AID - LAM13393 [pii]
AID - 10.1111/lam.13393 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2020 Dec;71(6):605-610. doi: 10.1111/lam.13393. Epub 2020 
      Oct 4.