PMID- 32966875
OWN - NLM
STAT- MEDLINE
DCOM- 20210517
LR  - 20211203
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Print)
IS  - 1096-4959 (Linking)
VI  - 250
DP  - 2020 Dec
TI  - Characterization of the hypoxia-inducible factor-1 pathway in hearts of Antarctic 
      notothenioid fishes.
PG  - 110505
LID - S1096-4959(20)30099-3 [pii]
LID - 10.1016/j.cbpb.2020.110505 [doi]
AB  - The ability of Antarctic notothenioid fishes to mount a robust molecular response 
      to hypoxia is largely unknown. The transcription factor, hypoxia-inducible 
      factor-1 (HIF-1), a heterodimer of HIF-1alpha and HIF-1beta subunits, is the master 
      regulator of oxygen homeostasis in most metazoans. We sought to determine if, in 
      the hearts of Antarctic notothenioids, HIF-1 is activated and functional in 
      response to either an acute heat stress or hypoxia. The red-blooded Notothenia 
      coriiceps and the hemoglobinless icefish, Chaenocephalus aceratus, were exposed 
      to their critical thermal maximum (CT(MAX)) or hypoxia (5.0 +/- 0.3 mg of O(2) 
      L(-1)) for 2 h. Additionally, N. coriiceps was exposed to 2.3 +/- 0.3 mg of O(2) 
      L(-1) for 12 h, and red-blooded Gobionotothen gibberifrons was exposed to both 
      levels of hypoxia. Levels of HIF-1alpha were quantified in nuclei isolated from heart 
      ventricles using western blotting. Transcript levels of genes involved in 
      anaerobic metabolism, and known to be regulated by HIF-1, were quantified by 
      real-time PCR, and lactate levels were measured in heart ventricles. Protein 
      levels of HIF-1alpha increase in nuclei of hearts of N. coriiceps and C. aceratus in 
      response to exposure to CT(MAX) and in hearts of N. coriiceps exposed to severe 
      hypoxia, yet mRNA levels of anaerobic metabolic genes do not increase in any 
      species, nor do lactate levels increase, suggesting that HIF-1 does not stimulate 
      metabolic remodeling in hearts of notothenioids under these conditions. Together, 
      these data suggest that Antarctic notothenioids may be vulnerable to hypoxic 
      events, which are likely to increase with climate warming.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - O'Brien, K M
AU  - O'Brien KM
AD  - Institute of Arctic Biology, Fairbanks, Alaska, United States of America. 
      Electronic address: kmobrien@alaska.edu.
FAU - Rix, A S
AU  - Rix AS
AD  - Institute of Arctic Biology, Fairbanks, Alaska, United States of America.
FAU - Grove, T J
AU  - Grove TJ
AD  - Department of Biology, Valdosta State University, Valdosta, GA 31698, United 
      States of America.
FAU - Sarrimanolis, J
AU  - Sarrimanolis J
AD  - Institute of Arctic Biology, Fairbanks, Alaska, United States of America.
FAU - Brooking, A
AU  - Brooking A
AD  - Institute of Arctic Biology, Fairbanks, Alaska, United States of America.
FAU - Roberts, M
AU  - Roberts M
AD  - Institute of Arctic Biology, Fairbanks, Alaska, United States of America.
FAU - Crockett, E L
AU  - Crockett EL
AD  - Department of Biological Sciences, Ohio University, Athens, OH 45701, United 
      States of America.
LA  - eng
GR  - P20 GM103395/GM/NIGMS NIH HHS/United States
GR  - RL5 GM118990/GM/NIGMS NIH HHS/United States
GR  - TL4 GM118992/GM/NIGMS NIH HHS/United States
GR  - UL1 GM118991/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20200920
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Antarctic Regions
MH  - Cell Hypoxia
MH  - Cell Nucleus/metabolism
MH  - Heat-Shock Response
MH  - Hypoxia-Inducible Factor 1/*metabolism
MH  - Lactic Acid/metabolism
MH  - Perciformes/*metabolism/physiology
MH  - Protein Transport
PMC - PMC7680639
MID - NIHMS1634997
OTO - NOTNLM
OT  - Anaerobic metabolism
OT  - Antarctic fishes
OT  - Heat stress
OT  - Hypoxia
COIS- Disclosures No conflicts of interest, financial or otherwise, are declared by the 
      authors.
EDAT- 2020/09/24 06:00
MHDA- 2021/05/18 06:00
PMCR- 2021/12/01
CRDT- 2020/09/23 20:05
PHST- 2020/08/01 00:00 [received]
PHST- 2020/09/09 00:00 [revised]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/09/24 06:00 [pubmed]
PHST- 2021/05/18 06:00 [medline]
PHST- 2020/09/23 20:05 [entrez]
PHST- 2021/12/01 00:00 [pmc-release]
AID - S1096-4959(20)30099-3 [pii]
AID - 10.1016/j.cbpb.2020.110505 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2020 Dec;250:110505. doi: 
      10.1016/j.cbpb.2020.110505. Epub 2020 Sep 20.