PMID- 32967115
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201028
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 9
IP  - 9
DP  - 2020 Sep 21
TI  - Bio-Guided Fractionation Driven by In Vitro alpha-Amylase Inhibition Assays of 
      Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic 
      Activity.
LID - 10.3390/plants9091242 [doi]
LID - 1242
AB  - Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by unpaired 
      blood glycaemia maintenance. T2DM can be treated by inhibiting carbohydrate 
      hydrolyzing enzymes (alpha-amylases and alpha-glucosidases) to decrease postprandial 
      hyperglycemia. Acarbose and voglibose are inhibitors used in clinical practice. 
      However, these drugs are associated with unpleasant gastrointestinal side 
      effects. This study explores new alpha-amylase inhibitors deriving from plant 
      volatile specialized metabolites. Sixty-two essential oils (EOs) from different 
      plant species and botanical families were subjected to alpha-amylase in vitro 
      enzymatic assay and chemically characterized using gas chromatography coupled to 
      mass spectrometry. Several EOs were found to be potential alpha-amylase inhibitors, 
      and Eucalyptus radiata, Laurus nobilis, and Myristicafragrans EOs displayed 
      inhibitory capacities comparable to that of the positive control (i.e., 
      acarbose). A bio-guided fractionation approach was adopted to isolate and 
      identify the active fractions/compounds of Eucalyptus radiata and Myristica 
      fragrans EOs. The bio-guided fractionation revealed that EOs alpha-amylase inhibitory 
      activity is often the result of antagonist, additive, or synergistic interactions 
      among their bioactive constituents and led to the identification of 1,8-cineole, 
      4-terpineol, alpha-terpineol, alpha-pinene, and beta-pinene as bioactive compounds, also 
      confirmed when they were tested singularly. These results demonstrate that EO 
      oils are a promising source of potential alpha-amylase inhibitors.
FAU - Capetti, Francesca
AU  - Capetti F
AUID- ORCID: 0000-0002-1024-7088
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
FAU - Cagliero, Cecilia
AU  - Cagliero C
AUID- ORCID: 0000-0003-3512-6124
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
FAU - Marengo, Arianna
AU  - Marengo A
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
FAU - Bicchi, Carlo
AU  - Bicchi C
AUID- ORCID: 0000-0003-1782-7357
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
FAU - Rubiolo, Patrizia
AU  - Rubiolo P
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
FAU - Sgorbini, Barbara
AU  - Sgorbini B
AUID- ORCID: 0000-0001-6848-1304
AD  - Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 
      9, 10125 Turin, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200921
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC7569863
OTO - NOTNLM
OT  - Eucalyptus radiata
OT  - Myristica fragrans
OT  - bio-guided fractionation
OT  - essential oil
OT  - alpha-amylase inhibition
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/09/25 06:00
MHDA- 2020/09/25 06:01
PMCR- 2020/09/21
CRDT- 2020/09/24 01:01
PHST- 2020/08/21 00:00 [received]
PHST- 2020/09/17 00:00 [revised]
PHST- 2020/09/19 00:00 [accepted]
PHST- 2020/09/24 01:01 [entrez]
PHST- 2020/09/25 06:00 [pubmed]
PHST- 2020/09/25 06:01 [medline]
PHST- 2020/09/21 00:00 [pmc-release]
AID - plants9091242 [pii]
AID - plants-09-01242 [pii]
AID - 10.3390/plants9091242 [doi]
PST - epublish
SO  - Plants (Basel). 2020 Sep 21;9(9):1242. doi: 10.3390/plants9091242.