PMID- 32967761
OWN - NLM
STAT- MEDLINE
DCOM- 20201117
LR  - 20211204
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 36
IP  - 9
DP  - 2020 Sep
TI  - [Knockdown of Kruppel like factor 4 (KLF4) promotes RAW264.7 macrophages into M1 
      polarization].
PG  - 782-787
AB  - Objective To investigate the effects of Kruppel like factor 4 (KLF4) gene 
      knockdown on the polarization of RAW264.7 macrophages. Methods KLF4 knockdown 
      lentiviral vector was constructed by RNA interfering. The lentiviral vector was 
      transfected into RAW264.7 cells to realize stable KLF4 gene silencing in RAW264.7 
      cells. Interleukin-4 (IL-4) was used to stimulate macrophages in wild type group, 
      KLF4 knockdown group and negative control group. The mRNA expression of inducible 
      nitric oxide synthase (iNOS), IL-1beta, tumor necrosis factor alpha (TNF-alpha) and Arg1, 
      IL-10, transforming growth factor beta (TGF-beta) of the cells was detected by reverse 
      transcription-PCR. Immunocytochemical staining was used to detect and localize 
      iNOS and Arg1 protein in RAW264.7 cells. Results Levels of iNOS and IL-1beta mRNA in 
      RAW264.7 cells were significantly raised, while levels of Arg1, IL-10 and TGF-beta 
      mRNA were significantly reduced after KLF4 gene knockdown. Levels of KLF4, Arg1, 
      IL-10 and TGF-beta mRNA went up, while the relative levels of iNOS, IL-1beta and TNF-alpha 
      mRNA went down in wild-type RAW264.7 cells after IL-4 intervention. After shKLF4 
      group was intervened by IL-4, levels of iNOS, IL-1beta and TNF-alpha mRNA in shKLF4 
      group (lentivirus group) were lower than those in wild-type group and higher than 
      those in negative control group. Levels of Arg1, IL-10 and TGF-beta mRNA in shKLF4 
      group after IL-4 treatment were higher than those in wild-type group, while Arg1 
      and IL-10 were lower than those in negative control group. Compared with 
      wide-type group, the expression of iNOS protein significantly decreased, while 
      Arg1 protein significantly increased in shKLF4 group 12 hours after IL-4 
      treatment. Conclusion Knockdown of KLF4 promotes the polarization of RAW264.7 
      macrophages into M1 as well as inhibits their polarization into M2.
FAU - Sun, Liang
AU  - Sun L
AD  - Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 
      300162, China. *Corresponding author, E-mail: sunliangys@sina.com.
FAU - Li, Xin
AU  - Li X
AD  - Third Medical Centre, Chinese People's Liberation Army General Hospital, Beijing 
      100039, China.
FAU - Ji, Wenjie
AU  - Ji W
AD  - Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 
      300162, China.
FAU - Wei, Luqing
AU  - Wei L
AD  - Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 
      300162, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Animals
MH  - Cell Polarity
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors
MH  - *Macrophages/metabolism
MH  - Mice
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - RAW 264.7 Cells
MH  - Transforming Growth Factor beta/genetics
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2020/09/25 06:00
MHDA- 2020/11/18 06:00
CRDT- 2020/09/24 05:26
PHST- 2020/09/24 05:26 [entrez]
PHST- 2020/09/25 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Sep;36(9):782-787.