PMID- 32968622
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220417
IS  - 2223-3652 (Print)
IS  - 2223-3660 (Electronic)
IS  - 2223-3652 (Linking)
VI  - 10
IP  - 4
DP  - 2020 Aug
TI  - Inhibition of ERK or Akt ameliorates intimal hyperplasia via up-regulation of 
      Cx37 and down-regulation of Cx43 in balloon injury rat model.
PG  - 658-666
LID - 10.21037/cdt-20-345 [doi]
AB  - BACKGROUND: Connexins (Cxs) are reported to participate in atherosclerosis 
      associated intimal hyperplasia (IH), while their function involved in the balloon 
      injury (BI) induced IH and restenosis is less reported. METHODS: Forty-eight male 
      Sprague-Dawley rats were randomly assigned to not injured (NI) group and BI 
      group, which were further administrated with ERK-inhibitor U0216 and 
      Akt-inhibitor MIK2206. Western blot and RT-PCR were utilized to detect the 
      expression of Cx30, Cx37, Cx40, and Cx43 at 6 hours, 24 hours, 7 days, and 14 
      days post-surgery. H&E staining and related intima area, media area, and luminal 
      area measurement were applied to indicate neointima formation and IH. ERK and Akt 
      phosphorylation levels and proliferating cell nuclear antigen (PCNA) 
      immunostaining were also detected. RESULTS: Among the four Cxs detected, Cx37 
      showed down-regulated, and Cx43 showed up-regulated temporal expression pattern 
      in BI rats with confirmed neointima formation. Up-regulated p-ERK (P<0.01) and 
      p-Akt (P<0.01) can be detected in BI rats compared with NI rats. Meanwhile, U0216 
      and MIK2206 can significantly reduce Cx43 expression and increase CX37 expression 
      accompanied with reduced neointima formation and PCNA staining (P<0.05 or P<0.01) 
      in BI rats. CONCLUSIONS: ERK or Akt inhibition can alleviate BI-induced IH via 
      up-regulation of Cx37 and down-regulation of Cx43.
CI  - 2020 Cardiovascular Diagnosis and Therapy. All rights reserved.
FAU - Pan, Lemen
AU  - Pan L
AD  - Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, China.
FAU - Ni, Haizhen
AU  - Ni H
AD  - Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, China.
FAU - Jin, Wenxu
AU  - Jin W
AD  - Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, China.
FAU - Su, Xiang
AU  - Su X
AD  - Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Cardiovasc Diagn Ther
JT  - Cardiovascular diagnosis and therapy
JID - 101601613
PMC - PMC7487390
OTO - NOTNLM
OT  - Akt
OT  - Cx37
OT  - Cx43
OT  - ERK
OT  - intimal hyperplasia (IH)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at http://dx.doi.org/10.21037/cdt-20-345). The authors have no 
      conflicts of interest to declare.
EDAT- 2020/09/25 06:00
MHDA- 2020/09/25 06:01
PMCR- 2020/08/01
CRDT- 2020/09/24 05:34
PHST- 2020/09/24 05:34 [entrez]
PHST- 2020/09/25 06:00 [pubmed]
PHST- 2020/09/25 06:01 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - cdt-10-04-658 [pii]
AID - 10.21037/cdt-20-345 [doi]
PST - ppublish
SO  - Cardiovasc Diagn Ther. 2020 Aug;10(4):658-666. doi: 10.21037/cdt-20-345.