PMID- 32971208
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20220531
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 65
DP  - 2021 Apr
TI  - Mechanisms underlying the production of chemokine CXCL11 in the reaction of renal 
      tubular epithelial cells with CD4(+) and CD8(+) T cells.
PG  - 101337
LID - S0966-3274(19)30148-0 [pii]
LID - 10.1016/j.trim.2020.101337 [doi]
AB  - AIM: To study the release mechanism of C-X-C motif chemokine 11 (CXCL11) and 
      other chemokines after the co-cultivation of CD4(+) and CD8(+) T cells with the 
      renal tubular epithelial cells (RTEC) in the process of allograft renal 
      transplantation rejection. METHODS: The Human CD4(+), CD8(+) T cells were 
      obtained from the blood of volunteers and kidney transplantation (Ktx) patients, 
      and co-cultured with renal tubular epithelial cells (RTEC) in vitro. RT-PCR was 
      run for detecting the mRNA transcription of CXCL11, IFN-induced protein of 10 
      (CXCL10), and IL-6 in cells after RTEC was stimulated with IFN-gamma or co-cultured 
      with CD4(+) and CD8(+) T cells. The concentration of CXCL11, CXCL10 and IL-6 in 
      the culture medium was detected by Multiplex Assay after RTEC was stimulated with 
      IFN-gamma or co-cultured with CD4(+) and CD8(+) T cells. IFN-gamma receptor antibody was 
      used for interfering with the above reaction and the blocking effect was 
      observed. Western blot was used for protein expression analysis. Finally, we 
      applied renal biopsies from kidney transplantation patients with and without 
      rejection to verify the results of the above experiments by using RT-PCR and 
      Western blot. RESULTS: The mRNA expression of CXCL11 and CXCL10 were 
      significantly increased after RTEC was stimulated with IFN-gamma or co-cultured with 
      CD4(+) and CD8(+) T cells. Multiplex Assay showed that the concentration of 
      CXCL11 and CXCL10 in the supernatant were significantly increased in a 
      time-dependence fashion after stimulation RTEC by IFN-gamma. Anti-IFN-gamma receptor1 
      (anti-IFN-gammaR1) antibody could reduce the production of CXCL11 and CXCL10 in this 
      situation. The concentration of CXCL11 and CXCL11 in the supernatant was 
      significantly increased with a time-dependent effect after the co-culture of 
      CD4(+) and CD8(+) T cells with RTEC. The anti-IFN-gammaR1 blocked this effect. Our 
      study showed that the expression levels of CXCL11 and CXCL10 were upgraded in the 
      biopsies of patients with renal transplant rejection comparatively to 
      pre-transplant biopsies, both at mRNA and protein levels. CONCLUSIONS: RTEC and T 
      cells can stimulate each other during the acute rejection of allogeneic kidney 
      transplantation and secret CXCL11，CXCL10 and other chemokines. IFN-gamma plays a key 
      role in this process.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Wang, Xiaoping
AU  - Wang X
AD  - Department of Nephrology, Shandong University & Jinan Central Hospital Affiliated 
      to Shandong First Medical University, Jinan 250000, China. Electronic address: 
      wang13395413385@163.com.
FAU - Wang, Dan
AU  - Wang D
AD  - Department of Nephrology, Shandong University & Jinan Central Hospital Affiliated 
      to Shandong First Medical University, Jinan 250000, China. Electronic address: 
      wangdan198566@163.com.
FAU - Wang, Xiao
AU  - Wang X
AD  - Department of Nephrology, Shandong University & Jinan Central Hospital Affiliated 
      to Shandong First Medical University, Jinan 250000, China. Electronic address: 
      1538253780@qq.com.
FAU - Wang, Xiaoqi
AU  - Wang X
AD  - Department of Cardiology, Shandong University & Jinan Central Hospital Affiliated 
      to Shandong First Medical University, Jinan 250000, China. Electronic address: 
      wangqiqi86@sina.com.
FAU - Sha, Ji-Chang
AU  - Sha JC
AD  - Department of Neurosurgery, Zhangqiu District People's Hospital, Jinan 250200, 
      China. Electronic address: wangqiqi86@sina.com.
FAU - Gao, Qingzhen
AU  - Gao Q
AD  - Department of Nephrology, Shandong University & Jinan Central Hospital Affiliated 
      to Shandong First Medical University, Jinan 250000, China. Electronic address: 
      qingzhengaogao@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200921
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (CXCL11 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL11)
RN  - 0 (Chemokine CXCL9)
SB  - IM
MH  - CD4-Positive T-Lymphocytes
MH  - *CD8-Positive T-Lymphocytes
MH  - *Chemokine CXCL10
MH  - Chemokine CXCL11
MH  - Chemokine CXCL9
MH  - Epithelial Cells
MH  - Humans
OTO - NOTNLM
OT  - Allograft renal transplantation rejection
OT  - Chemokine
OT  - IFN-inducible T cell chemoattractant
OT  - Renal tubular epithelial cells
EDAT- 2020/09/25 06:00
MHDA- 2022/01/14 06:00
CRDT- 2020/09/24 20:10
PHST- 2019/10/15 00:00 [received]
PHST- 2020/09/15 00:00 [revised]
PHST- 2020/09/19 00:00 [accepted]
PHST- 2020/09/25 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2020/09/24 20:10 [entrez]
AID - S0966-3274(19)30148-0 [pii]
AID - 10.1016/j.trim.2020.101337 [doi]
PST - ppublish
SO  - Transpl Immunol. 2021 Apr;65:101337. doi: 10.1016/j.trim.2020.101337. Epub 2020 
      Sep 21.