PMID- 32973567
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 11
DP  - 2020
TI  - Potential Application of Human beta-Defensin 4 in Dental Pulp Repair.
PG  - 1077
LID - 10.3389/fphys.2020.01077 [doi]
LID - 1077
AB  - When pulp tissue is damaged by caries or trauma, vital pulp therapy (VPT) can 
      help preserve the pulp tissue for long-term retention of teeth. However, the 
      choice of pulp capping agent used in VPT is important for the successful 
      preservation of the pulp tissue. Here we investigated the expression and 
      biological function of human beta-defensin 4 (HBD4) in dental pulp stem cells (DPSC) 
      and explored its potential as a pulp capping agent. We examined the expression of 
      HBD4 in DPSC in vitro using qPCR and immunofluorescence staining. We also looked 
      at the effect of HBD4 on inflammatory factors in lipopolysaccharide 
      (LPS)-stimulated DPSC, and its effects on mineralizing cell phenotype 
      differentiation, via qPCR and western blot. Finally, we examined the ability of 
      HBD4 to promote the restoration of the pulp-dentin complex in vivo, using male 
      Wistar rats with reversible pulpitis. We found HBD4 was highly expressed in DPSC 
      stimulated by TNF-alpha and IL-1alpha. HBD4 down-regulated the expression of inflammatory 
      mediators (i.e., IL-1alpha, IL-1beta, IL-6, TNF-alpha) in LPS-stimulated DPSC, and 
      suppressed MAPK activity and the NF-kappaB pathway. HBD4 also enhanced the 
      differentiation of DPSC into osteoblasts or odontoblasts, potentially by 
      modulating the Notch pathway. Furthermore, HBD4 controlled the degree of pulp 
      inflammation in a rat model of reversible pulpitis and induced the formation of 
      restorative dentin. Together our findings indicate HBD4 may be a useful pulp 
      capping agent for use in VPT.
CI  - Copyright (c) 2020 Zhai, Yuan, Zhao, Ge and Wang.
FAU - Zhai, Yue
AU  - Zhai Y
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Yuan, Xiaojing
AU  - Yuan X
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Zhao, Yuming
AU  - Zhao Y
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Ge, Lihong
AU  - Ge L
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200821
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7472722
OTO - NOTNLM
OT  - anti-inflammatory
OT  - dental pulp stem cells
OT  - human beta-defensin 4
OT  - stem cell differentiation
OT  - vital pulp therapy
EDAT- 2020/09/26 06:00
MHDA- 2020/09/26 06:01
PMCR- 2020/08/21
CRDT- 2020/09/25 06:01
PHST- 2020/05/28 00:00 [received]
PHST- 2020/08/05 00:00 [accepted]
PHST- 2020/09/25 06:01 [entrez]
PHST- 2020/09/26 06:00 [pubmed]
PHST- 2020/09/26 06:01 [medline]
PHST- 2020/08/21 00:00 [pmc-release]
AID - 10.3389/fphys.2020.01077 [doi]
PST - epublish
SO  - Front Physiol. 2020 Aug 21;11:1077. doi: 10.3389/fphys.2020.01077. eCollection 
      2020.