PMID- 32973841
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Cytogenetic Analysis and Molecular Marker Development for a New Wheat-Thinopyrum 
      ponticum 1J(s) (1D) Disomic Substitution Line With Resistance to Stripe Rust and 
      Powdery Mildew.
PG  - 1282
LID - 10.3389/fpls.2020.01282 [doi]
LID - 1282
AB  - Thinopyrum ponticum (2n = 10x = 70), a member of the tertiary gene pool of wheat 
      (Triticum aestivum L.), harbors many biotic and abiotic stress resistance genes. 
      CH10A5, a novel disomic substitution line from a cross of T. aestivum cv. 7182 
      and Th. ponticum, was characterized by cytogenetic identification, in situ 
      hybridization, molecular marker analysis, and morphological investigation of 
      agronomic traits and disease resistance. Cytological observations showed that 
      CH10A5 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. 
      Genome in situ hybridization (GISH) analysis indicated that two of its 
      chromosomes came from the J(s) genome of Th. ponticum, and wheat 15K array 
      mapping and fluorescence in situ hybridization (FISH) revealed that chromosome 1D 
      was absent from CH10A5. Polymorphic analysis of molecular markers indicated that 
      the pair of alien chromosomes belonged to homoeologous group one, designated as 
      1J(s). Thus, CH10A5 was a wheat-Th. ponticum 1J(s) (1D) disomic substitution 
      line. Field disease resistance trials demonstrated that the introduced Th. 
      ponticum chromosome 1J(s) was probably responsible for resistance to both stripe 
      rust and powdery mildew at the adult stage. Based on specific-locus amplified 
      fragment sequencing (SLAF-seq), 507 STS molecular markers were developed to 
      distinguish chromosome 1J(s) genetic material from that of wheat. Of these, 49 
      STS markers could be used to specifically identify the genetic material of Th. 
      ponticum. CH10A5 will increase the resistance gene diversity of wheat breeding 
      materials, and the markers developed here will permit further tracing of 
      heterosomal chromosome fragments in the future.
CI  - Copyright (c) 2020 Wang, Cao, Zhang, Wang, Chen, Wang, Zhang, Wang and Ji.
FAU - Wang, Yanzhen
AU  - Wang Y
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Cao, Qiang
AU  - Cao Q
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Zhang, Junjie
AU  - Zhang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Wang, Siwen
AU  - Wang S
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Chen, Chunhuan
AU  - Chen C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Wang, Changyou
AU  - Wang C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Wang, Yajuan
AU  - Wang Y
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
FAU - Ji, Wanquan
AU  - Ji W
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A&F University, Yangling, China.
AD  - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, 
      Ministry of Agriculture, Yangling, China.
LA  - eng
PT  - Journal Article
DEP - 20200821
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7472378
OTO - NOTNLM
OT  - Bgt SLAF-seq
OT  - Pst
OT  - Thinopyrum ponticum
OT  - wheat 15K array
OT  - wide hybridization
EDAT- 2020/09/26 06:00
MHDA- 2020/09/26 06:01
PMCR- 2020/01/01
CRDT- 2020/09/25 06:02
PHST- 2020/03/06 00:00 [received]
PHST- 2020/08/06 00:00 [accepted]
PHST- 2020/09/25 06:02 [entrez]
PHST- 2020/09/26 06:00 [pubmed]
PHST- 2020/09/26 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.01282 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 Aug 21;11:1282. doi: 10.3389/fpls.2020.01282. eCollection 
      2020.