PMID- 32977980 OWN - NLM STAT- MEDLINE DCOM- 20210629 LR - 20210629 IS - 1879-0097 (Electronic) IS - 0109-5641 (Linking) VI - 36 IP - 11 DP - 2020 Nov TI - Biomimetic remineralization of artificial caries dentin lesion using Ca/P-PILP. PG - 1397-1406 LID - S0109-5641(20)30228-1 [pii] LID - 10.1016/j.dental.2020.08.017 [doi] AB - OBJECTIVE: To assess the ultrastructural change of demineralized dentin collagen during calcium phosphate polymer-induced liquid precursor (Ca/P-PILP) mediated remineralization process and to evaluate the biomimetic remineralization potential of high concentration Ca/P-PILP at demineralized artificial caries dentin lesion, additionally to investigate the bond interfacial integrity as well as the bonding strength of the biomimetic remineralized artificial caries dentin lesion. METHODS: Demineralized dentin collagen of 5 mum thick was biomimetically remineralized with low, medium concentration Ca/P-PILP for 10 days and high concentration Ca/P-PILP for 10, 15, 20 days. Artificial caries dentin lesion at a thickness of 150 +/- 50 mum was biomimetically remineralized with high concentration Ca/P-PILP for 20 days. The biomimetic remineralization of demineralized dentin collagen was observed by scanning electron microscopy (SEM). The biomimetic remineralization intensity and depth of artificial caries dentin lesion was assessed by Electron Probe Micro Analyzer (EPMA). The bonding interfacial integrity between remineralized artificial caries dentin and composite resin was observed by Swept-source optical coherence tomography (SS-OCT) and the bonding strength of remineralized artificial caries dentin was evaluated by micro-tensile bond strength analysis (muTBS). RESULTS: Solely PAA-PASP solution and solely saturated Ca/P solution can't achieve dentin collagen remineralization. Increased concentration of Ca/P-PILP and prolonged remineralization time can enhance the biomimetic remineralization intensity of demineralized dentin collagen. After treating with high concentration Ca/P-PILP, a 150 +/- 50 mum thick layer of demineralized artificial caries dentin lesion was not fully remineralized, and the biomimetic remineralization intensity reached up to 88.0%. Furthermore, a better bonding interfacial integrity with less microgap and increased bond strength at both baseline level and aging level were observed when artificial caries dentin lesion was biomimetically remineralized with high concentration Ca/P-PILP. SIGNIFICANCE: Biomimetic remineralization of demineralized caries dentin lesion promotes its clinical properties for resin composited adhesive restoration. CI - Copyright (c) 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. FAU - Chen, Ruitian AU - Chen R AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Jin, Runze AU - Jin R AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Li, Xin AU - Li X AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Leuven, Belgium. FAU - Fang, Xiaolin AU - Fang X AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Yuan, Ding AU - Yuan D AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Chen, Zhuo AU - Chen Z AD - Department of Endodontics, The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, Hangzhou, China. FAU - Yao, Shasha AU - Yao S AD - Center for Biomaterials and Biopathways Department of Chemistry Zhejiang University Hangzhou, Zhejiang, China. FAU - Tang, Ruikang AU - Tang R AD - Center for Biomaterials and Biopathways Department of Chemistry Zhejiang University Hangzhou, Zhejiang, China. FAU - Chen, Zhi AU - Chen Z AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: zhichen@whu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200922 PL - England TA - Dent Mater JT - Dental materials : official publication of the Academy of Dental Materials JID - 8508040 RN - 0 (Calcium Phosphates) RN - 0 (Dental Cements) RN - 0 (Polymers) RN - 9007-34-5 (Collagen) MH - *Biomimetics MH - Calcium Phosphates MH - Collagen MH - Dental Caries Susceptibility MH - Dental Cements MH - *Dentin MH - Polymers MH - Tooth Remineralization OTO - NOTNLM OT - Amorphous calcium phosphate OT - Artificial caries dentin lesion OT - Biomimetic remineralization OT - Bonding interfacial integrity OT - Calcium phosphate polymer-induced liquid precursor OT - Micro-tensile bond strength EDAT- 2020/09/27 06:00 MHDA- 2021/06/30 06:00 CRDT- 2020/09/26 05:23 PHST- 2020/02/21 00:00 [received] PHST- 2020/06/19 00:00 [revised] PHST- 2020/08/29 00:00 [accepted] PHST- 2020/09/27 06:00 [pubmed] PHST- 2021/06/30 06:00 [medline] PHST- 2020/09/26 05:23 [entrez] AID - S0109-5641(20)30228-1 [pii] AID - 10.1016/j.dental.2020.08.017 [doi] PST - ppublish SO - Dent Mater. 2020 Nov;36(11):1397-1406. doi: 10.1016/j.dental.2020.08.017. Epub 2020 Sep 22.