PMID- 32978771
OWN - NLM
STAT- MEDLINE
DCOM- 20210531
LR  - 20210531
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 58
IP  - 3
DP  - 2020
TI  - An overview of applications of CRISPR-Cas technologies in biomedical engineering.
PG  - 163-173
LID - 10.5603/FHC.a2020.0023 [doi]
AB  - Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one of the 
      major genome editing systems and allows changing DNA levels of an organism. Among 
      several CRISPR categories, the CRISPR-Cas9 system has shown a remarkable 
      progression rate over its lifetime. Recently, other tools including CRISPR-Cas12 
      and CRISPR-Cas13 have been introduced. CRISPR-Cas9 system has played a key role 
      in the industrial cell factory's production and improved our understanding of 
      genome function. Additionally, this system has been used as one of the major 
      genome editing systems for the diagnosis and treatment of several infectious and 
      non-infectious diseases. In this review, we discuss CRISPR biology, its 
      versatility, and its application in biomedical engineering.
FAU - Jamehdor, Saleh
AU  - Jamehdor S
AD  - Department of Virology, Faculty of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, Iran.
AD  - Department of Molecular Medicine, Institute of Medical Biotechnology, National 
      Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
AD  - Department of Biology, Faculty of Sciences, University of Sistan and Baluchestan, 
      Zahedan, Iran.
FAU - Zaboli, Kasra Arbabi
AU  - Zaboli KA
AD  - Department of Medical Biotechnology, School of Medicine, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
FAU - Naserian, Sina
AU  - Naserian S
AD  - INSERM UMR-S-MD 1197/Ministry of the Armed Forces, Biomedical Research Institute 
      of the Armed Forces (IRBA), Paul-Brousse Hospital Villejuif, and CTSA Clamart, 
      France.
AD  - SivanCell, Sivan Aryo Pharmed, Tehran, Iran.
FAU - Thekkiniath, Jose
AU  - Thekkiniath J
AD  - Fuller Laboratories, Fullerton, CA, USA.
FAU - Omidy, Honey Alef
AU  - Omidy HA
AD  - Department of Biochemistry and Molecular Biology, University of Southern 
      California, Los Angeles, CA, USA.
FAU - Teimoori, Ali
AU  - Teimoori A
AD  - Department of Virology, Faculty of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, Iran.
FAU - Johari, Behrooz
AU  - Johari B
AD  - Department of Medical Biotechnology, School of Medicine, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
FAU - Taromchi, Amir Hossein
AU  - Taromchi AH
AD  - Department of Medical Biotechnology, School of Medicine, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
FAU - Sasano, Yu
AU  - Sasano Y
AUID- ORCID: 0000-0002-6889-2143
AD  - Department of Applied Microbial Technology, Faculty of Biotechnology and Life 
      Science, Sojo University, Kumamoto, Japan.
FAU - Kaboli, Saeed
AU  - Kaboli S
AD  - Department of Medical Biotechnology, School of Medicine, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20200926
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
SB  - IM
MH  - Animals
MH  - Biomedical Engineering/*methods
MH  - *CRISPR-Cas Systems
MH  - Cell Engineering
MH  - Drug Discovery
MH  - Gene Editing/methods
MH  - Humans
MH  - Models, Biological
OTO - NOTNLM
OT  - CRISPR-Cas12
OT  - CRISPR-Cas13
OT  - CRISPR-Cas9
OT  - Genome editing
OT  - sgRNA
EDAT- 2020/09/27 06:00
MHDA- 2021/06/01 06:00
CRDT- 2020/09/26 05:31
PHST- 2020/07/22 00:00 [received]
PHST- 2020/09/17 00:00 [accepted]
PHST- 2020/09/15 00:00 [revised]
PHST- 2020/09/27 06:00 [pubmed]
PHST- 2021/06/01 06:00 [medline]
PHST- 2020/09/26 05:31 [entrez]
AID - VM/OJS/J/70103 [pii]
AID - 10.5603/FHC.a2020.0023 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2020;58(3):163-173. doi: 10.5603/FHC.a2020.0023. Epub 
      2020 Sep 26.