PMID- 32980469
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20210104
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 149
DP  - 2020 Dec
TI  - Immunopathogenesis of infectious bronchitis virus Q1 in specific pathogen free 
      chicks.
PG  - 104535
LID - S0882-4010(20)30901-3 [pii]
LID - 10.1016/j.micpath.2020.104535 [doi]
AB  - The immunopathogenesis of avian coronavirus, infectious bronchitis virus (IBV) 
      Q1, was investigated in specific pathogen free chicks. Following infection, 
      chicks exhibited respiratory clinical signs and reduced body weight. 
      Oropharyngeal (OP) and cloacal (CL) swabs were collected at intervals and found 
      to be RT-PCR positive, with a greater number of partial-S1 amino acid changes 
      noted in CL swabs compared to OP swabs. In tissue samples, IBV viral load peaked 
      9 days post infection (dpi) in the trachea and kidneys, and 14 dpi in the 
      proventriculus. At 28 dpi, ELISA data showed that 63% of infected chicks 
      seroconverted. There was significantly higher mRNA up-regulation of IFN-alpha, TLR3, 
      MDA5, LITAF, IL-1beta and IL-6 in the trachea compared to the kidneys. Findings 
      presented here demonstrate that this Q1 isolate induces greater lesions and host 
      innate immune responses in chickens' tracheas compared to the kidneys.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Manswr, B
AU  - Manswr B
AD  - Institute of Infection and Global Health, University of Liverpool, Leahurst, UK; 
      Faculty of Veterinary Medicine, Diyala University, Iraq.
FAU - Ball, C
AU  - Ball C
AD  - Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.
FAU - Forrester, A
AU  - Forrester A
AD  - Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.
FAU - Chantrey, J
AU  - Chantrey J
AD  - Institute of Infection and Global Health, University of Liverpool, Leahurst, UK; 
      Institute of Veterinary Science, University of Liverpool, Leahurst, UK.
FAU - Ganapathy, K
AU  - Ganapathy K
AD  - Institute of Infection and Global Health, University of Liverpool, Leahurst, UK. 
      Electronic address: gana@liverpool.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20200924
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Body Weight
MH  - Chickens/*immunology
MH  - Coronavirus Infections/immunology/pathology/*veterinary/virology
MH  - Cytokines/blood/genetics/metabolism
MH  - Gene Expression
MH  - Immunity, Innate
MH  - Infectious bronchitis virus/genetics/*immunology/isolation & purification
MH  - Poultry Diseases/*immunology/pathology/*virology
MH  - Specific Pathogen-Free Organisms
MH  - Viral Load
OTO - NOTNLM
OT  - Avian coronavirus
OT  - Gene transcription
OT  - Immune responses
OT  - Infectious bronchitis virus
OT  - Pathogenesis
OT  - Specific pathogen free
EDAT- 2020/09/28 06:00
MHDA- 2021/01/05 06:00
CRDT- 2020/09/27 20:24
PHST- 2020/04/01 00:00 [received]
PHST- 2020/07/17 00:00 [revised]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/09/28 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
PHST- 2020/09/27 20:24 [entrez]
AID - S0882-4010(20)30901-3 [pii]
AID - 10.1016/j.micpath.2020.104535 [doi]
PST - ppublish
SO  - Microb Pathog. 2020 Dec;149:104535. doi: 10.1016/j.micpath.2020.104535. Epub 2020 
      Sep 24.