PMID- 3298062
OWN - NLM
STAT- MEDLINE
DCOM- 19870729
LR  - 20220310
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 55
IP  - 7
DP  - 1987 Jul
TI  - Discrepancy in assessment of phagosome-lysosome fusion with two lysosomal markers 
      in murine macrophages infected with Candida albicans.
PG  - 1663-7
AB  - Phagosome-lysosome fusion (P-LF) was studied in cultured mouse resident 
      peritoneal macrophages after phagocytosis of Candida albicans. The macrophages 
      were labeled with acridine orange (AO), the electronopaque colloidal Thorotrast, 
      or both markers. After phagocytosis of heat-killed C. albicans, both markers were 
      delivered to more than 95% of phagosomes. After ingestion of viable C. albicans 
      by labeled cells, delivery of AO to phagosomes was highly suppressed (90%), and 
      yet Thorotrast delivery was almost universal. After phagocytosis and 60 min of 
      incubation, about 10 to 20% of the yeasts were killed, and a similar fraction of 
      phagosomes was stained by the fluorescent marker. The evidence from Thorotrast 
      transfer and assessment of yeast viability indicates that C. albicans largely 
      resists intracellular killing by resident macrophages in the face of entirely 
      uninhibited P-LF. We infer that AO must transfer to nearly all of the phagosomes 
      but that it is evidently recognizable only in those in which the yeasts have been 
      killed or possibly severely injured. This conclusion constitutes yet another 
      limitation in the usefulness of AO for studying P-LF.
FAU - Mor, N
AU  - Mor N
FAU - Goren, M B
AU  - Goren MB
LA  - eng
GR  - AI08401/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Polyelectrolytes)
RN  - 0 (Polymers)
RN  - 0 (polyanions)
RN  - 9XA7X17UQC (Thorium Dioxide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Candida albicans/*physiology
MH  - Cytotoxicity, Immunologic
MH  - Hydrogen-Ion Concentration
MH  - Lysosomes/*physiology
MH  - Macrophages/microbiology/*physiology/ultrastructure
MH  - Membrane Fusion
MH  - Mice
MH  - Phagosomes/*physiology
MH  - Polyelectrolytes
MH  - Polymers
MH  - Thorium Dioxide
PMC - PMC260575
EDAT- 1987/07/01 00:00
MHDA- 1987/07/01 00:01
PMCR- 1987/07/01
CRDT- 1987/07/01 00:00
PHST- 1987/07/01 00:00 [pubmed]
PHST- 1987/07/01 00:01 [medline]
PHST- 1987/07/01 00:00 [entrez]
PHST- 1987/07/01 00:00 [pmc-release]
AID - 10.1128/iai.55.7.1663-1667.1987 [doi]
PST - ppublish
SO  - Infect Immun. 1987 Jul;55(7):1663-7. doi: 10.1128/iai.55.7.1663-1667.1987.