PMID- 32981128
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20211015
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 105
IP  - 1
DP  - 2021 Jan
TI  - Effect of genistein on the gene and protein expressions of CXCL-12 and EGR-1 in 
      the rat ovary.
PG  - 191-197
LID - 10.1111/jpn.13448 [doi]
AB  - The effect of genistein (GEN) on the gene expression level of stromal 
      cell-derived factor-1/CXCL-12 and early growth response gene-1 was studied in 
      ovarian tissue of young and initially ageing (early stages in the ageing process) 
      female rats. Forty, young female Sprague Dawley (SD) rats of 2-3 months old 
      (200 +/-20 g) and forty, initially ageing female SD rats of 10-12 months 
      (490 +/- 20 g) old were selected. According to the weight, rats were divided into 
      control group, low-dose group (L), medium-dose group (M) and a high-dose group 
      (H) and were given 15, 30 and 60 mg/kg GEN respectively. The positive control 
      (Oestrogen) group was given 0.5 mg/kg diethylstilbestrol. The treatment lasted 
      for 30 days. The mRNA expression of C-X-C motif chemokine ligand 12 (CXCL-12) and 
      early growth response factor-1 (EGR-1) was measured by real-time PCR, and protein 
      expression of EGR-1 was detected by Western blot. When compared to the negative 
      control group (NC), the ovary/body weight ratio in the young rats decreased in 
      the GEN group, but the difference was not significant. Similarly, compared with 
      NC, the ovary/body weight ratio in the initially ageing rats also decreased with 
      the increase in GEN concentration, but the decrease was significant in M and H 
      groups (p < .01). The administration of GEN enhanced both the gene and protein 
      expression levels of CXCL-12 and EGR-1 in the ovary. Pearson's correlation 
      analysis showed a synergistic effect between CXCL-12 and EGR-1. Thus, we conclude 
      that the effect of GEN on CXCL-12 and EGR-1 in the initially ageing group was 
      obvious than that in the younger group.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Zhang, Tao
AU  - Zhang T
AD  - College of Medical Laboratory Science and Technology, Harbin Medical 
      University-Daqing, Daqing, Heilongjiang Province, China.
FAU - Chi, Xiao-Xing
AU  - Chi XX
AUID- ORCID: 0000-0003-1771-5638
AD  - College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, 
      Heilongjiang Province, China.
AD  - Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang 
      Province, Daqing, Heilongjiang Province, China.
FAU - Kong, Fan-Xiu
AU  - Kong FX
AD  - College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, 
      Heilongjiang Province, China.
FAU - Chu, Xiao-Li
AU  - Chu XL
AD  - College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, 
      Heilongjiang Province, China.
LA  - eng
GR  - 81673170/National Natural Science Foundation of China/
GR  - GY2017YD0215/Major Project of Applied Technology Research and Development Plan of 
      Heilongjiang Province/
PT  - Journal Article
DEP - 20200927
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (Estrogens)
RN  - DH2M523P0H (Genistein)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Estrogens
MH  - Female
MH  - *Genistein/pharmacology
MH  - *Ovary
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - C-X-C motif chemokine ligand 12
OT  - early growth response gene-1
OT  - genistein
OT  - initially ageing rat
OT  - ovary
OT  - young female rat
EDAT- 2020/09/28 06:00
MHDA- 2021/10/16 06:00
CRDT- 2020/09/27 20:39
PHST- 2020/01/21 00:00 [received]
PHST- 2020/08/20 00:00 [accepted]
PHST- 2020/09/28 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2020/09/27 20:39 [entrez]
AID - 10.1111/jpn.13448 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2021 Jan;105(1):191-197. doi: 10.1111/jpn.13448. 
      Epub 2020 Sep 27.