PMID- 32981897
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201031
IS  - 1674-8301 (Print)
IS  - 2352-4685 (Electronic)
IS  - 1674-8301 (Linking)
VI  - 34
IP  - 5
DP  - 2020 Jun 30
TI  - Exposure to environmental bisphenol A inhibits HTR-8/SVneo cell migration and 
      invasion.
PG  - 369-378
LID - 10.7555/JBR.34.20200013 [doi]
AB  - Environmental pollutants, such as bisphenol A (BPA) have recently been implicated 
      in the development of adverse birth outcomes. However, the underlying teratogenic 
      mechanisms remain unclear. We investigated the effects of BPA on the migration 
      and invasion of human primary extravillous trophoblast HTR-8/SVneo cells. Our 
      results indicated that BPA reduced cell migration and invasion. Moreover, it 
      altered the ratio of matrix metalloproteinases (MMPs) and tissue inhibitors of 
      MMPs (TIMPs) by downregulating MMP-2 and MMP-9, and upregulating TIMP-1 and 
      TIMP-2. Furthermore, BPA suppressed integrin beta1, integrin alpha5, and vimentin. 
      Interestingly, BPA-induced invasion was partially restored by G15, a membrane 
      G-protein-coupled estrogen receptor 30 antagonist. We further revealed that 42 
      proteins were differentially expressed by mass spectrometry analysis, which could 
      be divided into three categories based on gene ontology including biological 
      process, cellular component, and molecular function. These results suggest that 
      BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and 
      MMP-9, up-regulating TIMP-1 and TIMP-2, and suppressing adhesion molecules.
FAU - Wei, Pu
AU  - Wei P
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
AD  - Department of Obstetrics, the Affiliated Hangzhou Hospital of Nanjing Medical 
      University, Hangzhou, Zhejiang 310006, China.
FAU - Ru, Dongqing
AU  - Ru D
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
FAU - Li, Xiaoqian
AU  - Li X
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
FAU - Shi, Dongyan
AU  - Shi D
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
FAU - Zhang, Mingshun
AU  - Zhang M
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
FAU - Xu, Qing
AU  - Xu Q
AD  - Department of Gynecology, the Affiliated Obstetrics and Gynecology Hospital of 
      Nanjing Medical University, Nanjing, Jiangsu 210004, China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
AD  - Department of Biotherapy, the Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210011, China.
FAU - Wen, Shuang
AU  - Wen S
AD  - Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Biomed Res
JT  - Journal of biomedical research
JID - 101551157
PMC - PMC7540237
OTO - NOTNLM
OT  - HTR8/SVneo cells
OT  - bisphenol A
OT  - cell invasion
OT  - integrin beta1
EDAT- 2020/09/29 06:00
MHDA- 2020/09/29 06:01
PMCR- 2020/09/01
CRDT- 2020/09/28 05:35
PHST- 2020/09/28 05:35 [entrez]
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2020/09/29 06:01 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - jbr-34-5-369 [pii]
AID - 10.7555/JBR.34.20200013 [doi]
PST - ppublish
SO  - J Biomed Res. 2020 Jun 30;34(5):369-378. doi: 10.7555/JBR.34.20200013.