PMID- 32983130
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20240329
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - HSV-1 Modulates IL-6 Receptor Expression on Human Dendritic Cells.
PG  - 1970
LID - 10.3389/fimmu.2020.01970 [doi]
LID - 1970
AB  - Dendritic cells (DCs) are the guardians of the immune system since they are 
      located in the majority of peripheral tissues. In addition, they are crucial for 
      the induction of an effective immune response based on their unique capacity to 
      stimulate naive T cells. During co-evolution, the human pathogen herpes simplex 
      virus type 1 (HSV-1) has evolved several immune evasion mechanisms in order to 
      subvert the host's immune system especially by targeting DC biology and function. 
      Here we demonstrate that HSV-1 infection influences the IL-6 receptor (IL6R) 
      expression both on protein and mRNA levels in/on human monocyte-derived mature 
      DCs (mDCs). Surprisingly, reduced IL6R expression levels were also observed on 
      uninfected bystander mDCs. Mechanistically, we clearly show that HSV-1-derived 
      non-infectious light (L-) particles are sufficient to trigger IL6R regulation on 
      uninfected bystander mDCs. These L-particles lack the viral DNA-loaded capsid and 
      are predominantly produced during infection of mDCs. Our results show that the 
      deletion of the HSV-1 tegument protein vhs partially rescued the reduced IL6R 
      surface expression levels on/in bystander mDCs. Using a neutralizing antibody, 
      which perturbs the transfer of L-particles to bystander mDCs, was sufficient to 
      rescue the modulation of IL6R surface expression on uninfected bystander mDCs. 
      This study provides evidence that L-particles transfer specific viral proteins to 
      uninfected bystander mDCs, thereby negatively interfering with their IL6R 
      expression levels, however, to a lesser extend compared to H-particles. Due to 
      their immune-modulatory capacity, L-particles represent an elaborated approach of 
      HSV-1-mediated immune evasion.
CI  - Copyright (c) 2020 Birzer, Krawczyk, Drassner, Kuhnt, Muhl-Zurbes, Heilingloh, 
      Steinkasserer and Popella.
FAU - Birzer, Alexandra
AU  - Birzer A
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Krawczyk, Adalbert
AU  - Krawczyk A
AD  - Institute for Virology, University Hospital Essen, University of Duisburg-Essen, 
      Essen, Germany.
AD  - Department of Infectious Diseases, University Hospital Essen, University of 
      Duisburg-Essen, Essen, Germany.
FAU - Drassner, Christina
AU  - Drassner C
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Kuhnt, Christine
AU  - Kuhnt C
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Muhl-Zurbes, Petra
AU  - Muhl-Zurbes P
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Heilingloh, Christiane Silke
AU  - Heilingloh CS
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
AD  - Department of Infectious Diseases, University Hospital Essen, University of 
      Duisburg-Essen, Essen, Germany.
FAU - Steinkasserer, Alexander
AU  - Steinkasserer A
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Popella, Linda
AU  - Popella L
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200826
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Biomarkers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-6)
SB  - IM
MH  - Biomarkers
MH  - Bystander Effect
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*metabolism
MH  - *Gene Expression Regulation
MH  - Herpes Simplex/genetics/immunology/metabolism/virology
MH  - Herpesvirus 1, Human/isolation & purification/*physiology
MH  - Host-Pathogen Interactions/*genetics/immunology
MH  - Humans
MH  - Immunomodulation
MH  - Monocytes/immunology/metabolism
MH  - RNA, Messenger/genetics
MH  - Receptors, Interleukin-6/*genetics/metabolism
MH  - Virus Replication
PMC - PMC7479228
OTO - NOTNLM
OT  - HSV-1
OT  - IL-6 receptor
OT  - L-particles
OT  - bystander cells
OT  - mature dendritic cells
OT  - vhs
EDAT- 2020/09/29 06:00
MHDA- 2021/05/01 06:00
PMCR- 2020/01/01
CRDT- 2020/09/28 05:40
PHST- 2020/05/15 00:00 [received]
PHST- 2020/07/21 00:00 [accepted]
PHST- 2020/09/28 05:40 [entrez]
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.01970 [doi]
PST - epublish
SO  - Front Immunol. 2020 Aug 26;11:1970. doi: 10.3389/fimmu.2020.01970. eCollection 
      2020.