PMID- 32985108
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210502
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 28
IP  - 11
DP  - 2020 Nov
TI  - A Novel Approach to Assess Metabolic Flexibility Overnight in a Whole-Body Room 
      Calorimeter.
PG  - 2073-2077
LID - 10.1002/oby.22982 [doi]
AB  - OBJECTIVE: This study aimed to investigate a novel approach for determining the 
      effects of energy-standardized dinner meals (high-fat and low-fat) on respiratory 
      exchange ratio (RER) dynamics and metabolic flexibility. METHODS: Using a 
      randomized crossover study design, energy expenditure, RER, and macronutrient 
      oxidation rates were assessed in response to a single dinner meal during an 
      overnight stay in a whole-body room calorimeter. Eight healthy adults completed 
      two overnight chamber stays while fed either a high-fat (60% fat, 20% 
      carbohydrate [CHO], 20% protein; food quotient [FQ] = 0.784) or low-fat (20% fat, 
      60% CHO, 20% protein; FQ = 0.899) dinner containing 40% of daily energy 
      requirements. RESULTS: Following the low-fat meal, CHO oxidation first increased 
      before decreasing, resulting in a 12-hour RER:FQ ratio close to 1.0 
      (0.986 +/- 0.019, P = 0.06) and therefore resulting in a 12-hour equilibrated fat 
      balance (29 +/- 76 kcal/12 hours). Following the high-fat meal, participants had a 
      RER:FQ ratio above 1.0 (1.061 +/- 0.017, P < 0.01), resulting in a significant 
      positive 12-hour fat balance of 376 +/- 142 kcal/12 hours. Various RER trajectory 
      parameters were significantly different following the high-fat and low-fat meals. 
      CONCLUSIONS: This proof-of-concept study provides an alternative approach to 
      quantify metabolic flexibility in response to a high-fat dinner and it can be 
      used to derive indexes of metabolic flexibility, such as the 12-hour RER:FQ ratio 
      or the 12-hour fat balance.
CI  - (c) 2020 The Obesity Society.
FAU - McDougal, David H
AU  - McDougal DH
AUID- ORCID: 0000-0003-4243-4897
AD  - Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
FAU - Marlatt, Kara L
AU  - Marlatt KL
AUID- ORCID: 0000-0001-7721-7900
AD  - Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
FAU - Beyl, Robbie A
AU  - Beyl RA
AUID- ORCID: 0000-0002-8582-744X
AD  - Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
FAU - Redman, Leanne M
AU  - Redman LM
AUID- ORCID: 0000-0002-7267-7374
AD  - Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
FAU - Ravussin, Eric
AU  - Ravussin E
AUID- ORCID: 0000-0003-2129-547X
AD  - Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
LA  - eng
GR  - P30 DK072476/DK/NIDDK NIH HHS/United States
GR  - T32 DK064584/DK/NIDDK NIH HHS/United States
GR  - U54 GM104940/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200927
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Calorimetry/*methods
MH  - Cross-Over Studies
MH  - Energy Metabolism/*physiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Metabolic Flux Analysis/*methods
MH  - Young Adult
PMC - PMC7644592
MID - NIHMS1614545
COIS- Disclosures: The authors declare no conflicts of interest.
EDAT- 2020/09/29 06:00
MHDA- 2021/03/31 06:00
PMCR- 2021/05/01
CRDT- 2020/09/28 05:51
PHST- 2020/02/26 00:00 [received]
PHST- 2020/07/15 00:00 [revised]
PHST- 2020/07/16 00:00 [accepted]
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/09/28 05:51 [entrez]
PHST- 2021/05/01 00:00 [pmc-release]
AID - 10.1002/oby.22982 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2020 Nov;28(11):2073-2077. doi: 10.1002/oby.22982. Epub 
      2020 Sep 27.