PMID- 32985213
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20210106
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 64
IP  - 3
DP  - 2020
TI  - A new single nucleopolyhedrovirus isolate from Cabbage looper, Trichoplusia ni 
      (Hubner) (Lepidoptera: Noctuidae): Detection, characterization and virulence.
PG  - 344-351
LID - 10.4149/av_2020_312 [doi]
AB  - This study was focused on the detection, characterization and virulence of a new 
      baculovirus isolate from the larvae of cabbage looper, Trichoplusia ni (Hubner) 
      (Lepidoptera: Noctuidae). T. ni is a polyphagous pest, and it has cosmopolitan 
      distribution. An infected T. ni larvae was collected from a cabbage field in 
      Turkey. Scanning electron microscopy studies showed the presence of typical 
      occlusion bodies (OBs) with average size of 0.76 to 1.46 microm in the collected 
      larvae. Since the virions have single nucleocapsid within the envelopes, the 
      isolate was named as Trichoplusia ni single nucleopolyhedrovirus Turkey isolate 
      (TrniSNPV- TR). The total genome of the TrniSNPV-TR was determined as 122.9 kb in 
      size. Sequence analysis of the amplified late expression factor 8 (lef8), late 
      expression factor 9 (lef9) and polyhedrin (polh) genes showed that the virus is a 
      new isolate of nucleopolyhedroviruses and close to Trichoplusia ni SNPV isolates 
      mentioned in the literature. However, this is the first study for the detection 
      and characterization of a baculovirus from T. ni in Eurasian region. Insecticidal 
      activities of the TrniSNPV-TR isolate (106 OBs/ml-1) against neonate, 3rd and 5th 
      instar larvae of T. ni and Helicoverpa armigera showed 98%-91%, 91%-87% and 
      65%-60% mortalities, respectively, within 14 days. LC50 of TrniSNPV-TR was 
      determined as 2x103-9x103, 3x104-7x104 and 1x105-2x105 OBs/ml on neonate, 3rd and 
      5th instar larvae, respectively. All these results showed that, TrniSNPV-TR has 
      good potential to be utilized as a bio-pesticide against T. ni larvae in the 
      future. Keywords: baculovirus; nucleopolyhedrovirus; Trichoplusia ni; 
      TrniSNPV-TR; biological control.
FAU - Eroglu, G B
AU  - Eroglu GB
FAU - Nalcacioglu, R
AU  - Nalcacioglu R
FAU - Nakai, M
AU  - Nakai M
FAU - Demirbag, Z
AU  - Demirbag Z
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
SB  - IM
MH  - Animals
MH  - Brassica
MH  - Larva
MH  - *Moths/virology
MH  - *Nucleopolyhedroviruses/classification/isolation & purification
MH  - Turkey
MH  - Virulence
EDAT- 2020/09/29 06:00
MHDA- 2021/01/07 06:00
CRDT- 2020/09/28 08:42
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/09/28 08:42 [entrez]
AID - 10.4149/av_2020_312 [doi]
PST - ppublish
SO  - Acta Virol. 2020;64(3):344-351. doi: 10.4149/av_2020_312.