PMID- 32985919
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20210607
IS  - 1744-7593 (Electronic)
IS  - 1742-5247 (Linking)
VI  - 18
IP  - 2
DP  - 2021 Feb
TI  - Physical transfection technologies for macrophages and dendritic cells in 
      immunotherapy.
PG  - 229-247
LID - 10.1080/17425247.2021.1828340 [doi]
AB  - INTRODUCTION: Dendritic cells (DCs) and macrophages, two important antigen 
      presenting cells (APCs) of the innate immune system, are being explored for the 
      use in cell-based cancer immunotherapy. For this application, the therapeutic 
      potential of patient-derived APCs is increased by delivering different types of 
      functional macromolecules, such as mRNA and pDNA, into their cytosol. Compared to 
      the use of viral and non-viral delivery vectors, physical intracellular delivery 
      techniques are known to be more straightforward, more controllable, faster and 
      generate high delivery efficiencies. AREAS COVERED: This review starts with 
      electroporation as the most traditional physical transfection method, before 
      continuing with the more recent technologies such as sonoporation, nanowires and 
      microfluidic cell squeezing. A description is provided of each of those 
      intracellular delivery technologies with their strengths and weaknesses, 
      especially paying attention to delivery efficiency and safety profile. EXPERT 
      OPINION: Given the common use of electroporation for the production of 
      therapeutic APCs, it is recommended that more detailed studies are performed on 
      the effect of electroporation on APC fitness, even down to the genetic level. 
      Newer intracellular delivery technologies seem to have less impact on APC 
      functionality but further work is needed to fully uncover their suitability to 
      transfect APCs with different types of macromolecules.
FAU - Harizaj, Aranit
AU  - Harizaj A
AUID- ORCID: 0000-0001-6949-9610
AD  - Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, 
      Ghent, Belgium.
FAU - De Smedt, Stefaan C
AU  - De Smedt SC
AD  - Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, 
      Ghent, Belgium.
FAU - Lentacker, Ine
AU  - Lentacker I
AD  - Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, 
      Ghent, Belgium.
FAU - Braeckmans, Kevin
AU  - Braeckmans K
AD  - Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, 
      Ghent, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20201019
PL  - England
TA  - Expert Opin Drug Deliv
JT  - Expert opinion on drug delivery
JID - 101228421
SB  - IM
MH  - *Dendritic Cells
MH  - *Electroporation
MH  - Humans
MH  - Immunotherapy
MH  - Macrophages
MH  - Transfection
OTO - NOTNLM
OT  - Cancer immunotherapy
OT  - antigens
OT  - cell engineering
OT  - dendritic cells
OT  - electroporation
OT  - innate immunity
OT  - mRNA
OT  - macrophages
OT  - microfluidic cell squeezing
OT  - nanowires
OT  - pDNA
OT  - sonoporation
OT  - transfection
EDAT- 2020/09/29 06:00
MHDA- 2021/06/08 06:00
CRDT- 2020/09/28 12:19
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2020/09/28 12:19 [entrez]
AID - 10.1080/17425247.2021.1828340 [doi]
PST - ppublish
SO  - Expert Opin Drug Deliv. 2021 Feb;18(2):229-247. doi: 
      10.1080/17425247.2021.1828340. Epub 2020 Oct 19.