PMID- 32986908
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 52
IP  - 11
DP  - 2020 Dec
TI  - Intratunical injection of human urine-derived stem cells derived exosomes 
      prevents fibrosis and improves erectile function in a rat model of Peyronie's 
      disease.
PG  - e13831
LID - 10.1111/and.13831 [doi]
AB  - We aimed to evaluate the effects of intratunical injection of exosomes derived 
      from human urine-derived stem cells (USC-exo) on plaque formation and erectile 
      function in a transforming growth factor-beta1 (TGF-beta1) induced Peyronie's disease 
      (PD) rat model. Twenty-four SD rats were randomly assigned equally to three 
      groups: (I) Sham group (50 mul phosphate-buffered saline [PBS] injected into the 
      tunica albuginea [TA]), (II) PD group (0.5 mug TGF-beta1 in 50 mul PBS injected into 
      the TA) and (III) USC-exo group (0.5 ug TGF-beta1 plus 100 mug USC-exo injected into 
      the TA at the same day). The maximum intracavernous pressure (ICP(max) ) and mean 
      arterial pressure (MAP) of each group were evaluated 4 weeks after injection. The 
      plaque formation, fibrosis, matrix metalloproteinases (MMPs) and tissue inhibitor 
      of MMPs (TIMPs) in the TA were evaluated. Four weeks after injection, USC-exo 
      group showed more significantly enhanced ICP(max) and ICP(max) /MAP than PD group 
      (p < .05). USC-exo could significantly ameliorate the TA fibrosis that could be 
      associated with the inhibition of transdifferentiation of fibroblasts into 
      myofibroblasts, decreased expression of TIMPs (TIMP-1, 2, 3) and increased 
      activity of MMPs (MMP-1, 3, 9) in the TA. According to these findings, USC-exo 
      can be a new candidate for the prevention of PD.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Yang, Qiyun
AU  - Yang Q
AUID- ORCID: 0000-0002-8077-9223
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
FAU - Chen, Wanmei
AU  - Chen W
AD  - Department of Anesthesiology, the First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Han, Dayu
AU  - Han D
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
FAU - Zhang, Chi
AU  - Zhang C
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
FAU - Xie, Yun
AU  - Xie Y
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
FAU - Sun, Xiangzhou
AU  - Sun X
AUID- ORCID: 0000-0002-8592-7854
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
FAU - Liu, Guihua
AU  - Liu G
AUID- ORCID: 0000-0002-1494-0883
AD  - Reproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun 
      Yat-Sen University, Guangzhou, China.
FAU - Deng, Chunhua
AU  - Deng C
AUID- ORCID: 0000-0002-4969-2875
AD  - Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, China.
LA  - eng
GR  - 81671449/the National Natural Science Foundation of China/
GR  - 81671834/the National Natural Science Foundation of China/
GR  - 8187111/the National Natural Science Foundation of China/
GR  - 2018A030310286/the Natural Science Foundation of Guangdong Province, China/
GR  - 18zxxt14/The Basic scienti fi c research project of Sun Yat-sen University/
PT  - Journal Article
DEP - 20200928
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - *Erectile Dysfunction/pathology/prevention & control
MH  - *Exosomes
MH  - Fibrosis
MH  - Humans
MH  - Male
MH  - *Penile Induration/pathology
MH  - Penis/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Stem Cells
MH  - Urine/cytology
OTO - NOTNLM
OT  - Peyronie's disease
OT  - exosomes
OT  - fibrosis
OT  - urine-derived stem cells
EDAT- 2020/09/29 06:00
MHDA- 2021/05/25 06:00
CRDT- 2020/09/28 17:13
PHST- 2020/07/17 00:00 [received]
PHST- 2020/08/08 00:00 [revised]
PHST- 2020/08/12 00:00 [accepted]
PHST- 2020/09/29 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2020/09/28 17:13 [entrez]
AID - 10.1111/and.13831 [doi]
PST - ppublish
SO  - Andrologia. 2020 Dec;52(11):e13831. doi: 10.1111/and.13831. Epub 2020 Sep 28.