PMID- 32987711
OWN - NLM
STAT- MEDLINE
DCOM- 20210225
LR  - 20210225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 19
DP  - 2020 Sep 24
TI  - Prognostic Significance of Stromal Periostin Expression in Non-Small Cell Lung 
      Cancer.
LID - 10.3390/ijms21197025 [doi]
LID - 7025
AB  - BACKGROUND: The microenvironment of solid tumours is significant in cancer 
      development and progression. The aim of this study was to determine periostin 
      (POSTN) expression by cancer-associated fibroblasts (CAFs) in non-small-cell lung 
      cancer (NSCLC), as well as to assess associations with clinicopathological 
      factors and prognosis. MATERIALS AND METHODS: Immunohistochemical analysis of 
      POSTN expression was performed on NSCLC (N = 700) and non-malignant lung tissue 
      (NMLT) (N = 110) using tissue microarrays. Laser capture microdissection (LCM) 
      for isolation of stromal and cancer cells of NSCLC was employed, and 
      subsequently, POSTN mRNA expression was detected by real-time PCR. 
      Immunofluorescence reaction and colocalisation analysis were performed by 
      confocal microscopy. RESULTS: Expression of POSTN in CAFs was significantly 
      higher in NSCLC and in the adenocarcinoma (AC) and squamous cell carcinoma (SCC) 
      subtypes compared to NMLT. POSTN expression in CAFs increased with clinical 
      cancer stage, grades (G) of malignancy, and lymph node involvement in NSCLC. 
      Higher POSTN expression in CAFs was an independent prognostic factor for overall 
      survival (OS). LCM confirmed significantly higher POSTN mRNA expression in the 
      stromal cells (CAFs) compared to the lung cancer cells. CONCLUSIONS: POSTN 
      produced by CAFs might be crucial for NSCLC progression and can be an independent 
      negative prognostic factor in NSCLC.
FAU - Ratajczak-Wielgomas, Katarzyna
AU  - Ratajczak-Wielgomas K
AUID- ORCID: 0000-0001-6175-2204
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Kmiecik, Alicja
AU  - Kmiecik A
AUID- ORCID: 0000-0002-6498-717X
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Grzegrzolka, Jedrzej
AU  - Grzegrzolka J
AUID- ORCID: 0000-0002-1548-279X
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Piotrowska, Aleksandra
AU  - Piotrowska A
AUID- ORCID: 0000-0003-4093-7386
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Gomulkiewicz, Agnieszka
AU  - Gomulkiewicz A
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Partynska, Aleksandra
AU  - Partynska A
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Pawelczyk, Konrad
AU  - Pawelczyk K
AUID- ORCID: 0000-0001-8275-7266
AD  - Department of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, 
      Poland.
AD  - Department of Thoracic Surgery, Lower Silesian Centre of Lung Diseases, 53-439 
      Wroclaw, Poland.
FAU - Nowinska, Katarzyna
AU  - Nowinska K
AUID- ORCID: 0000-0001-8316-8893
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Podhorska-Okolow, Marzenna
AU  - Podhorska-Okolow M
AD  - Division of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, 
      Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AUID- ORCID: 0000-0002-8292-1385
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
AD  - Department of Physiotherapy, University School of Physical Education, 51-612 
      Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20200924
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (POSTN protein, human)
SB  - IM
MH  - Adenocarcinoma/diagnosis/*metabolism
MH  - Aged
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Non-Small-Cell Lung/diagnosis/*metabolism
MH  - Carcinoma, Squamous Cell/diagnosis/*metabolism
MH  - Cell Adhesion Molecules/*metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lung Neoplasms/diagnosis/*metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Tumor Microenvironment
PMC - PMC7582720
OTO - NOTNLM
OT  - cancer
OT  - cancer-associated fibroblasts (CAFs)
OT  - non-small cell lung carcinoma
OT  - periostin
OT  - tumour microenvironment
COIS- The authors have no conflicts of interest to declare.
EDAT- 2020/09/30 06:00
MHDA- 2021/02/26 06:00
PMCR- 2020/10/01
CRDT- 2020/09/29 01:02
PHST- 2020/08/24 00:00 [received]
PHST- 2020/09/14 00:00 [revised]
PHST- 2020/09/19 00:00 [accepted]
PHST- 2020/09/29 01:02 [entrez]
PHST- 2020/09/30 06:00 [pubmed]
PHST- 2021/02/26 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - ijms21197025 [pii]
AID - ijms-21-07025 [pii]
AID - 10.3390/ijms21197025 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Sep 24;21(19):7025. doi: 10.3390/ijms21197025.