PMID- 32990222
OWN - NLM
STAT- MEDLINE
DCOM- 20201118
LR  - 20201118
IS  - 1673-4254 (Print)
IS  - 2663-0842 (Electronic)
IS  - 1673-4254 (Linking)
VI  - 40
IP  - 9
DP  - 2020 Sep 30
TI  - [Human umbilical cord mesenchymal stem cells differentiate into neuron-like cells 
      after induction with B27-supplemented serum-free medium].
PG  - 1340-1345
LID - 10.12122/j.issn.1673-4254.2020.09.18 [doi]
AB  - OBJECTIVE: To evaluate the capacity and efficiency of human umbilical cord 
      mesenchymal stem cells (HUCMSCs) to differentiate into neuron- like cells after 
      induction with B27- supplemented serum- free medium. METHODS: HUCMSCs at passage 
      4 were cultured for 14 days with serum-containing medium (SCM) (group A), SCM 
      supplemented with 20 ng/mL nerve growth factor (NGF) and 10 ng/mL basic 
      fibroblast growth factor (bFGF) (group B), serum-free medium (SFM) (group C), or 
      SFM supplemented with 20 ng/mL NGF and 10 ng/mL bFGF. The culture medium were 
      changed every 3 days and the growth of the neurospheres was observed using an 
      inverted microscope. The cell markers were analyzed with flow cytometry and the 
      expressions of nestin, neuron- specific enolase (NSE), neurofilament heavy 
      polypeptide (NEFH), and glial fibrillary acidic protein (GFAP) were quantified by 
      quantitative real-time PCR (qRT-PCR) and Western blotting. RESULTS: Before 
      induction, HUCMSCs expressed abundant mesenchymal stem cell surface markers 
      including CD29 (99.5%), CD44 (49.6%) and CD105 (77.7%). Neuron-like cells were 
      observed in the cultures on days 7, 10, and 14, and the cell differentiation was 
      the best in group D, followed by groups C, B and A. In all the 4 groups, the 
      cellular expressions of nestin and GFAP gradually lowered while those of NEFH and 
      NSE increased progressively. The expressions of GFAP, NEFH, nestin and NSE were 
      significantly different between group A and the other 3 groups (P < 0.001 or 
      0.05). CONCLUSIONS: B27-supplemented SFM effectively induces the differentiation 
      of HUCMSCs into neuron- like cells, and the supplementation with cytokines (NGF 
      and bFGF) strongly promotes the cell differentiation.
FAU - Li, Yunyi
AU  - Li Y
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
FAU - Yang, Jinpei
AU  - Yang J
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
FAU - Fu, Guo
AU  - Fu G
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
FAU - Zhou, Pan
AU  - Zhou P
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
FAU - Li, Zhizhong
AU  - Li Z
AD  - Shenhe Hospital Affiliated to Jinan University, Heyuan 517000, China.
FAU - Jiao, Genlong
AU  - Jiao G
AD  - First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
SB  - IM
MH  - Cells, Cultured
MH  - Dietary Supplements
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Neurons
MH  - Umbilical Cord
PMC - PMC7544586
OTO - NOTNLM
OT  - differentiation
OT  - human umbilical cord mesenchymal stem cells
OT  - neuron-like cells
OT  - serum-free medium
EDAT- 2020/09/30 06:00
MHDA- 2020/11/20 06:00
PMCR- 2020/09/20
CRDT- 2020/09/29 09:20
PHST- 2020/09/29 09:20 [entrez]
PHST- 2020/09/30 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
PHST- 2020/09/20 00:00 [pmc-release]
AID - nfykdxxb-40-9-1340 [pii]
AID - 10.12122/j.issn.1673-4254.2020.09.18 [doi]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2020 Sep 30;40(9):1340-1345. doi: 
      10.12122/j.issn.1673-4254.2020.09.18.