PMID- 3299045
OWN - NLM
STAT- MEDLINE
DCOM- 19870730
LR  - 20201209
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 21
IP  - 2
DP  - 1987 Mar-Apr
TI  - [Comparison of the conformation of RNA from phage MS2 and 16S rRNA. Interaction 
      with dyes specific for the secondary structure of native RNA and RNA subjected to 
      hydrolysis by nuclease S1].
PG  - 515-28
AB  - The interaction of ethidium bromide (EtBr) with double-stranded (ds), and 
      acridine orange (AO) with single-stranded (ss) fragments of 16S rRNA Escherichia 
      coli in a wide range of ionic strength, at various pH, Zn2+ ion concentrations 
      and partial hydrolysis by nuclease S1 was investigated. It was shown that about 
      90% of the RNA molecule is accessible to both dyes, when the ionic strength is 
      near of 0.01 (pH 7). Approximately half of the RNA becomes inaccessible to dyes, 
      when the ionic strength was increased up to 0.08-0.24 (pH 4.7-7), independent on 
      the presence of Zn2+ ions (10(-3) M). About a half of the ds-, and a quarter of 
      the ss-segments of the RNA, deduced from the secondary structure model were 
      protected from the interaction with EtBr and AO. The hydrolysis of about a half 
      of ss-segments upon addition of the Zn2+ (10(-3) M) ions did not affect the RNA 
      tertiary structure. The experimental data obtained confirm the idea of the 
      existence of some "nucleus" (or "nuclei") within the 16S rRNA molecule. The 
      "nucleus" seems to be inaccessible to the dyes and is very stable to heat 
      denaturation. It was supposed that this structure is organized by means of 
      interaction of some of the parallelly oriented ds-segments, as it was suggested 
      earlier for the phage MS2 RNA structure.
FAU - Borisova, O F
AU  - Borisova OF
FAU - Grechko, V V
AU  - Grechko VV
FAU - Kuznetsova, N V
AU  - Kuznetsova NV
FAU - Sakharova, N K
AU  - Sakharova NK
FAU - Timokhina, G I
AU  - Timokhina GI
LA  - rus
PT  - Comparative Study
PT  - Journal Article
TT  - Sravnenie prostranstvennoi organizatsii RNK faga MS2 i 16S rRNK. Vzaimodeistvie s 
      krasiteliami, spetsifichnymi ko vtorichnoi strukture, nativnoi RNK i posle ee 
      gidroliza nukleazoi S1.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Viral)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Coliphages/genetics
MH  - Escherichia coli/genetics
MH  - Ethidium/pharmacology
MH  - Hydrolysis
MH  - Kinetics
MH  - *Nucleic Acid Conformation
MH  - Nucleic Acid Denaturation
MH  - RNA, Double-Stranded/drug effects
MH  - *RNA, Ribosomal/drug effects
MH  - *RNA, Viral/drug effects
EDAT- 1987/03/01 00:00
MHDA- 1987/03/01 00:01
CRDT- 1987/03/01 00:00
PHST- 1987/03/01 00:00 [pubmed]
PHST- 1987/03/01 00:01 [medline]
PHST- 1987/03/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1987 Mar-Apr;21(2):515-28.