PMID- 32994092
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1873-4898 (Electronic)
IS  - 1477-5131 (Linking)
VI  - 16
IP  - 6
DP  - 2020 Dec
TI  - Expression of NGF, MCP-1, uroplakin III, and NOS in bladder urothelium after 
      partial urethral obstruction in rats.
PG  - 806.e1-806.e14
LID - S1477-5131(20)30540-4 [pii]
LID - 10.1016/j.jpurol.2020.09.013 [doi]
AB  - INTRODUCTION: Although several cytokines, chemokines, and growth factors have 
      been suggested to play a role in the development of bladder fibrosis and 
      functional changes, the mechanisms that are effective in the pathogenesis of 
      partial bladder outlet obstruction (pBOO)-induced bladder fibrosis are not well 
      understood. OBJECTIVE: We investigated the expressions of nerve growth factor 
      (NGF), monocyte chemoattractant protein-1 (MCP-1), uroplakin III (URPIII), 
      inducible nitric oxide synthase (iNOS), and endothelial NOS (eNOS) that may be 
      involved in fibrosis in rats with partial urethral obstruction for 1, 2 and 3 
      weeks, and the changes in the associated ischemic and inflammatory processes. 
      After 1, 2, and 3 weeks of pBOO, blood samples were collected for assessment of 
      renal function from the rats under anesthesia. The bladders were dissected for 
      the tissue antioxidant enzyme activities and lipid peroxidation, including 
      malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant status (TAS) 
      and total oxidant status (TOS). The immunohistochemical studies were performed. 
      Histopathologically, the number of urothelial layers was calculated and the 
      thickness of the detrusor smooth muscle and lamina propria were quantitatively 
      measured. Additionally, the edema and congestion in the submucosa were evaluated. 
      STUDY DESIGN: Twenty-eight male Sprague-Dawley rats were used in this study. 
      Three separate experimental groups of pBOO (1 week [n = 7], 2 weeks [n = 7], and 
      3 weeks [n = 7]) were created, with an additional sham-operated control group 
      (n = 7). RESULTS: The MDA levels increased in pBOO groups. The SOD values were 
      decreased in the pBOO group for 1 week, and higher in the 3-week pBOO group. The 
      TAS levels were increased in the 3 week pBOO group. The TOS levels increased in 
      the pBOO groups. The number of urothelial layers was decreased in pBOO groups. 
      The lamina propria, the smooth muscle thickness, edema and congestion were 
      increase in the 1 and 2 week pBOO groups. The NGF and MCP-1 expression was 
      increased in the 1-week and 2-week pBOO groups. The expression of URPIII in the 
      epithelium gradually increased in the pBOO groups. In the pBOO groups, iNOS 
      expression in the epithelium cells was significantly elevated. However, the eNOS 
      expression was also significantly increased in the 2 week pBOO group. CONCLUSION: 
      Our study shows that overexpression of immunohistochemical parameters together 
      with the negative effects of ischemic and inflammatory processes that subjected 
      to pBOO for 1, 2 and 3 weeks may play a potential role in detrusor fibrosis in 
      the rat bladders induced by pBOO. However, understanding of the 
      immunohistochemical parameters investigated in this experimental study is 
      limited, and further studies targeting their relationship to pBOO could help us 
      develop new strategies.
CI  - Copyright (c) 2020 Journal of Pediatric Urology Company. Published by Elsevier Ltd. 
      All rights reserved.
FAU - Ozturk, Hayrettin
AU  - Ozturk H
AD  - Bolu Abant Izzet Baysal University, Medical School, Pediatric Surgery, Bolu, 
      Turkey. Electronic address: ozturkhayrettin@hotmail.com.
FAU - Cetinkaya, Ayhan
AU  - Cetinkaya A
AD  - Bolu Abant Izzet Baysal University, Medical School, Physiology, Bolu, Turkey.
FAU - Duzcu, Selma Erdogan
AU  - Duzcu SE
AD  - Bolu Abant Izzet Baysal University, Medical School, Pathology, Bolu, Turkey.
FAU - Yis, Ozgur Mehmet
AU  - Yis OM
AD  - Bolu Abant Izzet Baysal University, Medical School, Biochemistry, Bolu, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20200917
PL  - England
TA  - J Pediatr Urol
JT  - Journal of pediatric urology
JID - 101233150
RN  - 0 (Chemokine CCL2)
RN  - 0 (Uroplakin III)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2
MH  - Male
MH  - Mucous Membrane
MH  - Muscle, Smooth
MH  - Nerve Growth Factor
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Urinary Bladder Neck Obstruction
MH  - Uroplakin III
MH  - *Urothelium
OTO - NOTNLM
OT  - Ischemic damage
OT  - MCP-1
OT  - NGF
OT  - NOS
OT  - Urethral obstruction
OT  - Uroplakin III
COIS- Disclosure of potential conflicts of interest None.
EDAT- 2020/10/01 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/09/30 05:50
PHST- 2019/09/26 00:00 [received]
PHST- 2020/09/04 00:00 [revised]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/10/01 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/09/30 05:50 [entrez]
AID - S1477-5131(20)30540-4 [pii]
AID - 10.1016/j.jpurol.2020.09.013 [doi]
PST - ppublish
SO  - J Pediatr Urol. 2020 Dec;16(6):806.e1-806.e14. doi: 10.1016/j.jpurol.2020.09.013. 
      Epub 2020 Sep 17.