PMID- 32994409
OWN - NLM
STAT- MEDLINE
DCOM- 20210113
LR  - 20210929
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Sep 29
TI  - Omidenepag, a non-prostanoid EP2 receptor agonist, induces enlargement of the 3D 
      organoid of 3T3-L1 cells.
PG  - 16018
LID - 10.1038/s41598-020-72538-x [doi]
LID - 16018
AB  - 2D and 3D cultures of 3T3-L1 cells were employed in a study of the effects of 
      Omidenepag (OMD), interacting with a non-prostanoid EP2 receptor, on 
      adipogenesis. Upon adipogenesis, the effects on lipid staining, the mRNA 
      expression of adipogenesis-related genes (Ppargamma, CEBPa, Ap2, and Glut4) and the 
      extracellular matrix (ECM) including collagen type 1, 4 and 6, and fibronectin, 
      and the size and physical property of 3D organoids were compared between groups 
      that had been treated with EP2 agonists (butaprost and OMD) and PGF2alpha. Upon 
      adipogenesis, these significantly suppressed lipid staining and the mRNA 
      expression of related genes. EP2 agonists and PGF2alpha influenced the mRNA 
      expression of ECM in different manners, and these effects were also different 
      between 2 and 3D cultures. Examining the physical properties by a microsqueezer 
      indicated that the solidity of the 3D organoids became significantly lowered upon 
      adipogenesis and these effects were not affected by EP2 agonists. In contrast, 3D 
      organoid stiffness was markedly enhanced by the presence of PGF2alpha. These 
      observations indicate that EP2 agonists affect the adipogenesis of 3T3-L1 cells 
      in different manners, as compared to PGF2alpha, suggesting that OMD may not induce 
      PGF2alpha related orbital fat atrophy, called the deepening of the upper eyelid 
      sulcus (DUES).
FAU - Ida, Yosuke
AU  - Ida Y
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Hikage, Fumihito
AU  - Hikage F
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan. fuhika@gmail.com.
FAU - Umetsu, Araya
AU  - Umetsu A
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Ida, Haruka
AU  - Ida H
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Ohguro, Hiroshi
AU  - Ohguro H
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20200929
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Pyrazoles)
RN  - 0 (Pyridines)
RN  - B7IN85G1HY (Dinoprost)
RN  - G0G0H52U6K (omidenepag isopropyl)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Cell Culture Techniques
MH  - Dinoprost/*pharmacology
MH  - Extracellular Matrix/drug effects/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Gene Regulatory Networks/drug effects
MH  - Glycine/*analogs & derivatives/pharmacology
MH  - Mice
MH  - Organ Size/drug effects
MH  - Organoids/chemistry/*cytology/drug effects
MH  - Pyrazoles/*pharmacology
MH  - Pyridines/*pharmacology
PMC - PMC7524797
COIS- The authors declare no competing interests.
EDAT- 2020/10/01 06:00
MHDA- 2021/01/14 06:00
PMCR- 2020/09/29
CRDT- 2020/09/30 06:07
PHST- 2020/06/30 00:00 [received]
PHST- 2020/09/02 00:00 [accepted]
PHST- 2020/09/30 06:07 [entrez]
PHST- 2020/10/01 06:00 [pubmed]
PHST- 2021/01/14 06:00 [medline]
PHST- 2020/09/29 00:00 [pmc-release]
AID - 10.1038/s41598-020-72538-x [pii]
AID - 72538 [pii]
AID - 10.1038/s41598-020-72538-x [doi]
PST - epublish
SO  - Sci Rep. 2020 Sep 29;10(1):16018. doi: 10.1038/s41598-020-72538-x.