PMID- 32994780
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1642-395X (Print)
IS  - 2299-0046 (Electronic)
IS  - 1642-395X (Linking)
VI  - 37
IP  - 4
DP  - 2020 Aug
TI  - Effect of Oreodoxa oleracea Jacq. pollen protein allergen in albino mice.
PG  - 566-571
LID - 10.5114/ada.2020.98286 [doi]
AB  - INTRODUCTION: Pollen trigger allergenic reactions in hypersensitive individuals 
      due to the presence of protein in their sporoderm. AIM: Pollen protein produced 
      by Oreodoxa oleracea was subjected to allergenicity test in mice in order to 
      determine its allergenic potential. MATERIAL AND METHODS: Pollen protein was 
      extracted using 0.02 M phosphate buffered saline and used to inoculate mice by 
      two subcutaneous and one intranasal injections weekly for 4 weeks. Blood samples 
      were obtained by retro-orbital bleedings, sera obtained were used in detecting 
      immunoglobulin E (IgE) by immunoperoxidase assay. Values of immune cell and IgE 
      elicited in mice were analysed using SPSS statistical package version 20. 
      RESULTS: Oreodoxa oleracea yielded 208 microg/ml pollen protein allergen. Inoculation 
      of pollen protein in mice produced dermatophytic allergic reactions which 
      physically presented as swelling, rashes and hair loss. Pollen protein skewed 
      basophil production and infiltration of lymphocytes by 1-62% and 58-99% 
      respectively in relation to controls. Histopathology analysis showed inflammation 
      within the lung parenchyma. CONCLUSIONS: The present study is the first study to 
      evaluate the allergenic potential of Oreodoxa oleracea in mice. The findings 
      revealed that Oreodoxa oleracea which is profoundly planted in pubic areas for 
      aesthetic purposes possess allergenic effect.
CI  - Copyright: (c) 2020 Termedia Sp. z o. o.
FAU - Ezikanyi, Dimphna Nneka
AU  - Ezikanyi DN
AD  - Palynology Unit, Department of Applied Biology, Faculty of Science, Ebonyi State 
      University, Abakaliki, Nigeria.
FAU - Oselebe, Happiness
AU  - Oselebe H
AD  - Department of Crop Science, Faculty of Agriculture, Ebonyi State University, 
      Abakaliki, Nigeria.
LA  - eng
PT  - Journal Article
DEP - 20200902
PL  - Poland
TA  - Postepy Dermatol Alergol
JT  - Postepy dermatologii i alergologii
JID - 101168357
PMC - PMC7507149
OTO - NOTNLM
OT  - Oreodoxa oleracea
OT  - allergy
OT  - immune cell
OT  - mice
OT  - pollen protein
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/01 06:00
MHDA- 2020/10/01 06:01
PMCR- 2020/08/01
CRDT- 2020/09/30 06:12
PHST- 2019/01/22 00:00 [received]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2020/09/30 06:12 [entrez]
PHST- 2020/10/01 06:00 [pubmed]
PHST- 2020/10/01 06:01 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - 98286 [pii]
AID - 10.5114/ada.2020.98286 [doi]
PST - ppublish
SO  - Postepy Dermatol Alergol. 2020 Aug;37(4):566-571. doi: 10.5114/ada.2020.98286. 
      Epub 2020 Sep 2.