PMID- 32995525
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201002
IS  - 2397-7000 (Electronic)
IS  - 2397-7000 (Linking)
VI  - 3
IP  - 1
DP  - 2018
TI  - Design of RNA hairpin modules that predictably tune translation in yeast.
PG  - ysy019
LID - 10.1093/synbio/ysy019 [doi]
LID - ysy019
AB  - Modular parts for tuning translation are prevalent in prokaryotic synthetic 
      biology but lacking for eukaryotic synthetic biology. Working in Saccharomyces 
      cerevisiae yeast, we here describe how hairpin RNA structures inserted into the 
      5' untranslated region (5'UTR) of mRNAs can be used to tune expression levels by 
      100-fold by inhibiting translation. We determine the relationship between the 
      calculated free energy of folding in the 5'UTR and in vivo protein abundance, and 
      show that this enables rational design of hairpin libraries that give predicted 
      expression outputs. Our approach is modular, working with different promoters and 
      protein coding sequences, and outperforms promoter mutation as a way to 
      predictably generate a library where a protein is induced to express at a range 
      of different levels. With this new tool, computational RNA sequence design can be 
      used to predictably fine-tune protein production for genes expressed in yeast.
CI  - (c) The Author(s) 2018. Published by Oxford University Press.
FAU - Weenink, Tim
AU  - Weenink T
AD  - Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
AD  - Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
FAU - van der Hilst, Jelle
AU  - van der Hilst J
AD  - Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
AD  - Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
FAU - McKiernan, Robert M
AU  - McKiernan RM
AD  - Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
AD  - Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
FAU - Ellis, Tom
AU  - Ellis T
AD  - Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
AD  - Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
LA  - eng
PT  - Journal Article
DEP - 20181013
PL  - England
TA  - Synth Biol (Oxf)
JT  - Synthetic biology (Oxford, England)
JID - 101728857
PMC - PMC7445769
OTO - NOTNLM
OT  - RNA secondary structure
OT  - gene expression
OT  - modularity
OT  - translation
OT  - yeast
EDAT- 2018/10/13 00:00
MHDA- 2018/10/13 00:01
PMCR- 2018/10/13
CRDT- 2020/09/30 06:15
PHST- 2020/09/30 06:15 [entrez]
PHST- 2018/10/13 00:00 [pubmed]
PHST- 2018/10/13 00:01 [medline]
PHST- 2018/10/13 00:00 [pmc-release]
AID - ysy019 [pii]
AID - 10.1093/synbio/ysy019 [doi]
PST - epublish
SO  - Synth Biol (Oxf). 2018 Oct 13;3(1):ysy019. doi: 10.1093/synbio/ysy019. 
      eCollection 2018.