PMID- 32998244
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201110
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 10
IP  - 10
DP  - 2020 Sep 28
TI  - Combined Immunofluorescence (IFA) and Fluorescence In Situ Hybridization (FISH) 
      Assays for Diagnosing Babesiosis in Patients from the USA, Europe and Australia.
LID - 10.3390/diagnostics10100761 [doi]
LID - 761
AB  - Apicomplexan parasites of the genus Babesia cause babesiosis in humans and 
      animals worldwide. Human babesiosis is a predominantly zoonotic disease 
      transmitted by hard ticks that is of increasing health concern in the USA and 
      many other countries. Microscopic examination of stained blood smears, detection 
      of serum antibodies by immunoassays and identification of parasite nucleic acid 
      in blood by qPCR and fluorescence in situ hybridization (FISH) are some methods 
      available for diagnosing babesiosis. This study investigated the use of a Babesia 
      genus-specific FISH test for detecting Babesia parasites in blood smears and 
      immunofluorescence assay (IFA) for detecting serum antibodies to Babesia duncani 
      and Babesia microti, two common species that cause human babesiosis in the USA. 
      The findings with clinical samples originating from USA, Australia, Europe and 
      elsewhere demonstrate that the parallel use of Babesia genus-specific FISH and 
      IFA tests for B. duncani and B. microti provides more useful diagnostic 
      information in babesiosis and that B. duncani infections are more widespread 
      globally than presently recognized.
FAU - Shah, Jyotsna S
AU  - Shah JS
AD  - ID-FISH Technology Inc., Milpitas, CA 95035, USA.
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Caoili, Eddie
AU  - Caoili E
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Patton, Marie Fe
AU  - Patton MF
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Tamhankar, Snehal
AU  - Tamhankar S
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Myint, Mu Mu
AU  - Myint MM
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Poruri, Akhila
AU  - Poruri A
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Mark, Olivia
AU  - Mark O
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Horowitz, Richard I
AU  - Horowitz RI
AD  - Hudson Valley Healing Arts Center, New York, NY 12538, USA.
AD  - HHS Subcommittee on Babesia and Tick-Borne Pathogens, US Department of Health and 
      Human Services, Washington, DC 20201, USA.
FAU - Ashbaugh, Alan D
AU  - Ashbaugh AD
AD  - College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA.
FAU - Ramasamy, Ranjan
AU  - Ramasamy R
AD  - ID-FISH Technology Inc., Milpitas, CA 95035, USA.
LA  - eng
PT  - Journal Article
DEP - 20200928
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC7650773
OTO - NOTNLM
OT  - Babesia duncani
OT  - Babesia microti
OT  - babesiosis
OT  - fluorescence in situ hybridization
OT  - immunofluorescence assay
OT  - laboratory diagnosis of babesiosis
COIS- J.S.S. owns stock and is chief executive of IGeneX and scientific director of 
      IDFISH; R.R. is an affiliate of IDFISH; O.M., E.C., M.M.M., M.F.P., S.T. and A.P. 
      are employees at IGeneX.
EDAT- 2020/10/02 06:00
MHDA- 2020/10/02 06:01
PMCR- 2020/09/28
CRDT- 2020/10/01 01:00
PHST- 2020/08/04 00:00 [received]
PHST- 2020/09/18 00:00 [revised]
PHST- 2020/09/27 00:00 [accepted]
PHST- 2020/10/01 01:00 [entrez]
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2020/10/02 06:01 [medline]
PHST- 2020/09/28 00:00 [pmc-release]
AID - diagnostics10100761 [pii]
AID - diagnostics-10-00761 [pii]
AID - 10.3390/diagnostics10100761 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2020 Sep 28;10(10):761. doi: 10.3390/diagnostics10100761.