PMID- 32998395
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201222
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 6
IP  - 4
DP  - 2020 Sep 28
TI  - Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for 
      Antibodies with Therapeutic Potential.
LID - 10.3390/jof6040193 [doi]
LID - 193
AB  - Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in 
      Latin America. It affects mainly male rural workers in impoverished regions, and 
      the therapy can last up to two years or use drugs that are very toxic. Given the 
      need for novel safe and effective approaches to treat PCM, we have been 
      developing monoclonal antibodies (mAbs) that could be used not only to block 
      specific fungal targets, but also modulate the host's antifungal immunity. In 
      this work we show the generation of and promising results with an mAb against 
      Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence 
      factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. 
      brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and 
      generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very 
      immunogenic and both the polyclonal serum and mAb were used in immunofluorescence 
      experiments, which showed binding of antibodies to the yeast cell surface. The 
      mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations 
      with J774.16 macrophage-like cells. Our results suggest that this mAb could serve 
      as the basis for new immunotherapy regimens for PCM.
FAU - Moura, Agata Nogueira D'Aurea
AU  - Moura AND
AUID- ORCID: 0000-0003-0480-5028
AD  - Institute of Tropical Medicine, Department of Dermatology, Faculty of Medicine, 
      University of Sao Paulo, Sao Paulo 05403-000, SP, Brazil.
FAU - Oliveira, Diane Sthefany Lima de
AU  - Oliveira DSL
AUID- ORCID: 0000-0002-2655-4672
AD  - Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, DF, 
      Brazil.
FAU - Paredes, Verenice
AU  - Paredes V
AD  - Karan Technologies Research and Development, Brasilia 70632-200, DF, Brazil.
AD  - Faculty of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil.
FAU - Rocha, Leticia Barboza
AU  - Rocha LB
AUID- ORCID: 0000-0001-7832-058X
AD  - Bacteriology Laboratory, Butantan Institute, Sao Paulo 05503-900, SP, Brazil.
FAU - Oliveira, Fabiana Freire Mende de
AU  - Oliveira FFM
AD  - Faculty of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil.
FAU - Lessa, Gustavo Meirelles
AU  - Lessa GM
AD  - Faculty of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil.
FAU - Riasco-Palacios, Juan Fernando
AU  - Riasco-Palacios JF
AUID- ORCID: 0000-0002-9170-1034
AD  - Faculty of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil.
FAU - Casadevall, Arturo
AU  - Casadevall A
AUID- ORCID: 0000-0002-9402-9167
AD  - Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School 
      of Public Health, Baltimore, MD 21205, USA.
FAU - Albuquerque, Patricia
AU  - Albuquerque P
AUID- ORCID: 0000-0001-8160-7784
AD  - Faculty of Ceilandia, University of Brasilia, Brasilia 72220-275, DF, Brazil.
FAU - Felipe, Maria Sueli Soares
AU  - Felipe MSS
AD  - Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, DF, 
      Brazil.
AD  - Graduate Program in Genomic Sciences and Biotechnology, Catholic University of 
      Brasilia, Brasilia 70790-160, DF, Brazil.
FAU - Piazza, Roxane Maria Fontes
AU  - Piazza RMF
AD  - Bacteriology Laboratory, Butantan Institute, Sao Paulo 05503-900, SP, Brazil.
FAU - Nicola, Andre Moraes
AU  - Nicola AM
AUID- ORCID: 0000-0001-8656-5835
AD  - Karan Technologies Research and Development, Brasilia 70632-200, DF, Brazil.
AD  - Faculty of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil.
AD  - Graduate Program in Genomic Sciences and Biotechnology, Catholic University of 
      Brasilia, Brasilia 70790-160, DF, Brazil.
LA  - eng
GR  - R01 HL059842/HL/NHLBI NIH HHS/United States
GR  - 0193.001048/2015/Fundacao de Apoio a Pesquisa do Distrito Federal/
GR  - 193.001.533/2016/Fundacao de Apoio a Pesquisa do Distrito Federal/
GR  - 437484/2018-1/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 2015/17178-2/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
PT  - Journal Article
DEP - 20200928
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC7712200
OTO - NOTNLM
OT  - HSP90
OT  - Paracoccidioides spp.
OT  - monoclonal antibody
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/10/02 06:00
MHDA- 2020/10/02 06:01
PMCR- 2020/09/28
CRDT- 2020/10/01 01:01
PHST- 2020/08/09 00:00 [received]
PHST- 2020/09/05 00:00 [revised]
PHST- 2020/09/10 00:00 [accepted]
PHST- 2020/10/01 01:01 [entrez]
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2020/10/02 06:01 [medline]
PHST- 2020/09/28 00:00 [pmc-release]
AID - jof6040193 [pii]
AID - jof-06-00193 [pii]
AID - 10.3390/jof6040193 [doi]
PST - epublish
SO  - J Fungi (Basel). 2020 Sep 28;6(4):193. doi: 10.3390/jof6040193.