PMID- 33000257
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20210921
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 44
IP  - 5
DP  - 2020 Nov
TI  - lncRNA RASSF8‑AS1 suppresses the progression of laryngeal squamous cell carcinoma 
      via targeting the miR‑664b‑3p/TLE1 axis.
PG  - 2031-2044
LID - 10.3892/or.2020.7771 [doi]
AB  - Long non‑coding (lnc)RNAs have been found to play a crucial role in tumor 
      progression. The present study aimed to investigate the association between 
      lncRNA RASSF8‑AS1 and laryngeal squamous cell carcinoma (LSCC) and the underlying 
      mechanisms. Reverse transcription‑quantitative PCR was used to measure the mRNA 
      expression level of RASSF8‑AS1, microRNA(miR)‑664b‑3p and transducin‑like 
      enhancer of split 1 (TLE1) in LSCC. The associations between RASSF8‑AS1 and 
      miR‑664b‑3p, and between miR‑664b‑3p and TLE1 were investigated using a dual 
      luciferase reporter assay, while the former was further verified using an RNA 
      immunoprecipitation (RIP) assay. The association between RASSF8‑AS1 and 
      miR‑664b‑3p on cell biological functions was investigated in vitro using MTS, 
      colony formation and Transwell assays. The RASSF8‑AS1 mRNA expression level was 
      decreased in LSCC cell lines and carcinoma tissues, while overexpression of 
      RASSF8‑AS1 reduced the migration, invasion and proliferation abilities of LSCC 
      cells. Furthermore, luciferase and RIP assays confirmed that RASSF8‑AS1 was a 
      competitive endogenous (ce)RNA by sponging miR‑664b‑3p to activate TLE1. 
      miR‑664b‑3p was negatively modulated by RASSF8‑AS1; however, TLE1 was positively 
      regulated by RASSF8‑AS1. Functionally, RASSF8‑AS1 acted as a ceRNA to upregulate 
      TLE1 by sponging miR‑664b‑3p. In conclusion, the RASSF8‑AS1/miR‑664b‑3p/TLE1 axis 
      acts by suppressing LSCC progression and may provide a novel insight for the 
      molecular mechanism of LSCC.
FAU - Liu, Tao
AU  - Liu T
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Meng, Wenxia
AU  - Meng W
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Cao, Huan
AU  - Cao H
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Chi, Weiwei
AU  - Chi W
AD  - Department of Otorhinolaryngology, The First Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050031, P.R. China.
FAU - Zhao, Lei
AU  - Zhao L
AD  - Department of Otorhinolaryngology, The Affiliated Hospital of Hebei University, 
      Baoding, Hebei 071000, P.R. China.
FAU - Cui, Weina
AU  - Cui W
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Yin, Huan
AU  - Yin H
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Wang, Baoshan
AU  - Wang B
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200916
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (MIRN664 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TLE1 protein, human)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Co-Repressor Proteins/*genetics
MH  - Computational Biology
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/diagnosis/*genetics/pathology/surgery
MH  - Laryngectomy
MH  - Larynx/pathology/surgery
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - RNA, Long Noncoding/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/diagnosis/*genetics/pathology/surgery
PMC - PMC7551431
OTO - NOTNLM
OT  - long non-coding RNA
OT  - RASSF8-AS1
OT  - laryngeal squamous cell carcinoma
OT  - competitive endogenous RNA
EDAT- 2020/10/02 06:00
MHDA- 2021/07/07 06:00
PMCR- 2020/09/16
CRDT- 2020/10/01 05:39
PHST- 2019/12/11 00:00 [received]
PHST- 2020/08/28 00:00 [accepted]
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
PHST- 2020/10/01 05:39 [entrez]
PHST- 2020/09/16 00:00 [pmc-release]
AID - or-44-05-2031 [pii]
AID - 10.3892/or.2020.7771 [doi]
PST - ppublish
SO  - Oncol Rep. 2020 Nov;44(5):2031-2044. doi: 10.3892/or.2020.7771. Epub 2020 Sep 16.