PMID- 33000764
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20211208
IS  - 1758-5090 (Electronic)
IS  - 1758-5082 (Linking)
VI  - 12
IP  - 4
DP  - 2020 Oct 1
TI  - Flow enhances phenotypic and maturation of adult rat liver organoids.
PG  - 045035
LID - 10.1088/1758-5090/abb538 [doi]
AB  - A biologically relevant in vitro model of hepatic microtissue would be a valuable 
      tool for the preclinical study of pharmacokinetics and metabolism. Although 
      considerable advances have been made in recent years in the establishment of 
      alternative in vitro culture systems that mimic liver tissue, generating an 
      effective liver model remains challenging. Specifically, existing model systems 
      still exhibit limited functions for hepatocellular differentiation potential and 
      cellular complexity. It is essential to improve the in vitro differentiation of 
      liver progenitor cells (LPCs) for disease modeling and preclinical 
      pharmatoxicological research. Here, we describe a rat liver organoid culture 
      system under in vivo-like steady-state flow conditions; this system is capable of 
      controlling the expansion and differentiation of rat liver organoids over 10-15 
      d. LPCs cultured in medium flow conditions become self-assembled liver organoids 
      that exhibit phenotypic and functional hepato-biliary modeling. In addition, 
      hepatocytes that are differentiated using liver organoids produced albumin and 
      maintained polygonal morphology, which is characteristic of mature hepatocytes.
FAU - Jung, Da Jung
AU  - Jung DJ
AD  - Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan 
      Medical Center, 88 Olympic-Ro, Songpa-Gu, Seoul 05505, Republic of Korea.
FAU - Byeon, Jae Hee
AU  - Byeon JH
AD  - Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan 
      Medical Center, 88 Olympic-Ro, Songpa-Gu, Seoul 05505, Republic of Korea.
AD  - Department of Biomedical Engineering, Asan Medical Institute of Convergence 
      Science and Technology, Asan Medical Center, University of Ulsan College of 
      Medicine, Seoul, Republic of Korea.
FAU - Jeong, Gi Seok
AU  - Jeong GS
AUID- ORCID: 0000-0002-2584-9129
AD  - Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan 
      Medical Center, 88 Olympic-Ro, Songpa-Gu, Seoul 05505, Republic of Korea.
AD  - Author to whom any correspondence should be addressed.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201001
PL  - England
TA  - Biofabrication
JT  - Biofabrication
JID - 101521964
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - *Hepatocytes
MH  - Liver
MH  - *Organoids
MH  - Rats
MH  - Stem Cells
EDAT- 2020/10/02 06:00
MHDA- 2021/10/27 06:00
CRDT- 2020/10/01 08:38
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2020/10/01 08:38 [entrez]
AID - 10.1088/1758-5090/abb538 [doi]
PST - epublish
SO  - Biofabrication. 2020 Oct 1;12(4):045035. doi: 10.1088/1758-5090/abb538.