PMID- 33001070
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210412
LR  - 20210412
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 12
IP  - 37
DP  - 2020 Oct 7
TI  - An electrochemical aptasensor-based Co(x)P-decorated porous carbon microspheres 
      and AuNRs labelled methylene blue as signal labels for the sensitive detection of 
      PCB77.
PG  - 4579-4587
LID - 10.1039/d0ay01462a [doi]
AB  - In this work, a simple, specific and ultra-sensitive electrochemical aptasensor 
      was successfully developed based on a novel signal reduction strategy for the 
      detection of polychlorinated biphenyls (PCB77). This aptasensor was prepared by 
      the electrodeposition of gold nanoparticles (AuNPs) on an Au electrode (AuE) 
      modified with cobalt phosphide (Co(x)P, a mixture of CoP and Co(2)P) decorated 
      porous carbon microspheres. In this study, the thiolated single-complementary DNA 
      (cDNA) was immobilized on the surface of the modified electrode via the Au-S 
      bond. Subsequently, the gold nanorod@methylene blue connection aptamer 
      (AuNRs@MB-Apt) signal labels were immobilized onto the modified electrode through 
      the principle of complementary base pairing. Further, the aptamer preferentially 
      binds to PCB77, decreasing the amount of AuNR@MB-Apt. The DPV current response 
      was related to the PCB77 concentration. Under the optimized experimental 
      conditions, a low detection limit of 5.9 x 10(-2) ng L(-1) and a wide linear 
      range of 1 x 10(-11) mg mL(-1) to 1 x 10(-4) mg mL(-1) (S/N = 3) for PCB77 were 
      achieved. Moreover, the proposed aptasensor offered high selectivity, stability 
      and reproducibility, indicating the broad potential application in environmental 
      monitoring.
FAU - Tian, Panpan
AU  - Tian P
AD  - School of Chemistry and Chemical Engineering, Henan University of Technology, 
      Lianhua Road 100#, Zhengzhou, 450001, Henan Province, People's Republic of China.
FAU - Zhang, Baozhong
AU  - Zhang B
FAU - Lv, Lina
AU  - Lv L
FAU - Xie, Lingling
AU  - Xie L
FAU - Chen, Hanyu
AU  - Chen H
FAU - He, Baoshan
AU  - He B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200904
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
SB  - IM
EDAT- 2020/10/02 06:00
MHDA- 2020/10/02 06:01
CRDT- 2020/10/01 12:13
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2020/10/02 06:01 [medline]
PHST- 2020/10/01 12:13 [entrez]
AID - 10.1039/d0ay01462a [doi]
PST - ppublish
SO  - Anal Methods. 2020 Oct 7;12(37):4579-4587. doi: 10.1039/d0ay01462a. Epub 2020 Sep 
      4.